Isolated Rat Lung
Isolated Rat Lung - Related Videos
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
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This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
07:36
Angiogenesis in the Ischemic Rat Lung
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Cited by 3 •
2013
The lung is perfused by both the systemic bronchial artery and pulmonary arteries. In most lung pathologies, it is the smaller systemic vasculature that shows robust neovascularization. Cessation of pulmonary blood flow promotes brisk bronchial angiogenesis. We provide surgical details of inducing left pulmonary artery ischemia that promotes bronchial neovascularization.
07:22
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
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Cited by 5 •
2014
The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
FAQs
Ventilation delivers air through the airways, while perfusion moves a physiological solution through the pulmonary vasculature. Maintaining these two controlled flows preserves key aspects of lung activity and allows investigators to examine gas exchange and vascular behavior separately from whole-body influences. This separation helps connect specific changes in airway or vascular conditions with measurable pulmonary responses.
Control over the experimental environment reduces the influence of processes occurring elsewhere in the body. Researchers can adjust conditions while observing the lung directly, making it easier to attribute changes in gas exchange, vascular responses, or tissue injury to the tested experimental factor. This precision supports clearer investigation of mechanisms involved in pulmonary function and disease.
Vascular responses show how the pulmonary circulation reacts under experimental conditions, whereas permeability measurements indicate how readily substances may pass through vascular barriers. Together, these observations help investigators study processes associated with pulmonary edema and tissue injury. The preparation therefore links changes in the vasculature with broader alterations in lung function and disease-related damage.
The preparation begins by removing the lungs from the animal and transferring them to controlled laboratory conditions. Researchers then maintain activity by ventilating the airways and perfusing the vasculature with a physiological solution. Once these systems are functioning, investigators can measure gas exchange, vascular responses, tissue injury, and other effects under the selected experimental conditions.
This preparation is useful when investigators need to examine pulmonary events without the confounding influence of the rest of the animal. It allows direct control of conditions affecting the lung and supports focused studies of respiratory biology, edema, inflammation, vascular permeability, and injury. It can also help evaluate drug or toxicant effects specifically at the pulmonary level.
Measurements from the preparation can reveal changes in gas exchange, pulmonary vascular responses, tissue injury, inflammation, and vascular permeability. Researchers can use these outcomes to clarify mechanisms of lung disease and to assess how drugs or toxicants affect pulmonary tissue. The model is therefore valuable for connecting controlled experimental exposures with specific functional or injury-related responses.