Isotopic Labeling
Isotopic Labeling - Related Videos
09:26
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
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Cited by 1 •
2017
This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.
11:53
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
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Cited by 38 •
2014
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
10:16
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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Cited by 39 •
2014
This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.
02:31
Elements: Chemical Symbols and Isotopes
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2020
A chemical symbol is an abbreviation used to indicate an element or an atom of an element. For example, the symbol for mercury is Hg. The same symbol is used to indicate one atom of mercury (microscopic domain) or to label a container of many atoms of the element mercury (macroscopic domain). Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
07:12
Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
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2024
Presented here is a method for screening lipid structures through stable isotope labeling by using their retention time, mobility, and fragmentation.
FAQs
The isotope choice determines what can be measured and how it can be detected. Stable forms support analysis without radioactivity, whereas radioactive forms provide highly sensitive signals. This distinction matters when a study emphasizes precise chemical measurement, or when it needs sensitive tracking of transport, localization, or molecular interactions in biological systems.
Cells incorporate labeled compounds into products during metabolism or biosynthesis. Researchers then examine where the label appears and whether the original compound has undergone chemical conversion. Following movement, conversion, and incorporation connects an input compound with the resulting cellular products, helping reveal how biological transformations proceed and which biomolecules receive specific components.
Label movement provides a measurable way to assess the flow of material through biological processes. In metabolism, the resulting labeling patterns can help quantify metabolic flux, while changes in labeled cellular components can indicate molecular turnover. These measurements extend beyond identifying products by showing how actively compounds move through or are replaced within biological systems.
A typical workflow introduces a labeled compound to cells or another biological system, allows incorporation during metabolism or biosynthesis, and then examines the resulting products or cellular components. Mass spectrometry can analyze labeled material, while autoradiography can detect radioactive signals. The selected readout depends on whether the experiment prioritizes chemical measurement or sensitive localization.
Isotopic labeling can identify biosynthetic pathways, determine the origins of cellular components, quantify metabolic flux, and measure molecular turnover. These uses address both pathway structure and activity: researchers can ask where a component came from, how it was produced, and how rapidly material moves through or is replaced within a biological system.
Mass spectrometry is suited to analyzing labeled compounds and products, particularly when stable-isotope approaches are preferred. Autoradiography detects radioactive labels and supports highly sensitive studies of movement or location. Together, these techniques connect labeling experiments with complementary outcomes, including chemical conversion, incorporation into biomolecules, transport, localization, and molecular interaction analysis.