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Chapters
- 00:05Title
- 01:19Protein Purification and Alkylation of Free Sulfhydryl Groups
- 02:59Protein Digestion, Reversed Phase Peptide Extraction, and Reductive Dimethylation Labeling
- 06:02Basic pH Reversed Phase Chromatography and STop and Go Extraction
- 08:17Microcapillary LC-MS/MS and Peptide Quantification
- 09:58Results: Accuracy, Precision, and Reproducibility of ReDi Labeling
- 11:24Conclusion
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
Tags
Quantitative ProteomicsReductive DimethylationStable Isotope LabelingMass SpectrometryProtein Expression DifferencesRegular/formaldehyde LabelingDeuterated LabelingSodium CyanoborohydrideMethyl TagsLC-MS/MS AnalysisIon Chromatogram Peak AreasSample PreparationReversed-phase Solid Phase ExtractionBasic PH Reversed-phase ChromatographyStageTip Peptide PurificationAdvantages Of ReDi LabelingLimitations Of ReDi LabelingNovel Applications Of ReDi Labeling
