Lectin Pathway
Lectin Pathway - Related Videos
09:25
Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
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Cited by 21 •
2011
An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.
20:23
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
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Cited by 13 •
2009
Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.
08:11
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
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Cited by 54 •
2016
We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.
01:27
C4 Pathway and CAM
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2019
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy. C4 Pathway The C4 pathway is used by plants such as...
10:25
In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
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Cited by 2 •
2015
This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.
FAQs
Activation begins when mannose-binding lectin or ficolins recognize specific carbohydrate patterns on a microbial surface. This recognition step provides the molecular trigger for recruiting their associated MASP-1 and MASP-2 enzymes, allowing the pathway to respond to pathogen-associated structures without relying on antibodies. The carbohydrate-binding step therefore determines which surfaces enter the complement response.
MASP-1 and MASP-2 act as the enzymatic link between surface recognition and complement activation. Once associated with pattern-recognition proteins bound to microbial carbohydrates, they cleave complement components C4 and C2. Those cleavage events produce the C4b2a C3 convertase, shifting the response from recognition to amplification of downstream complement effects.
C4b2a is important because it functions as the pathway’s C3 convertase, connecting early recognition to major effector outcomes. Its activity promotes generation of C3b, which supports opsonization, while also contributing to inflammatory signaling and membrane attack complex formation. Studying this conversion point helps explain how a recognition event can produce microbial elimination.
The lectin pathway can recognize microbial surfaces through mannose-binding lectin and ficolins without requiring antibodies. This contrasts with complement activation mechanisms that depend on antibody recognition. The distinction is biologically important because it shows how innate immune defenses can detect characteristic microbial carbohydrates directly, before antibody participation is required.
A pathway analysis can follow a defined sequence: carbohydrate recognition by mannose-binding lectin or ficolins, activation of associated MASP-1 and MASP-2, cleavage of C4 and C2, and formation of C4b2a. Researchers can then relate this activity to C3b-mediated opsonization, inflammatory signaling, and membrane attack complex formation to evaluate downstream consequences.
Deficiencies in lectin pathway components can reveal how impaired recognition or enzymatic activation affects innate immune defense. Research on these deficiencies helps clarify the pathway’s contribution to host protection and identifies mechanisms that may influence disease. The same knowledge can support investigation of complement-related biomarkers and approaches for modulating complement activity.
Because lectin pathway activation can promote inflammatory signaling as well as microbial clearance, researchers can examine whether altered pathway activity is associated with inflammatory disease mechanisms. Mapping recognition proteins, MASP-dependent cleavage, and downstream complement effects provides a framework for connecting molecular activation with disease-related inflammation and for evaluating complement-modulating therapeutic strategies.