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JoVE Journal
Neuroscience

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Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
 

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons

Article DOI: 10.3791/3200
September 15th, 2011

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Summary September 15th, 2011

An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.

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