Lentiviral Gene Delivery
Lentiviral Gene Delivery - Related Videos
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
02:30
Gene Delivery to Mouse Intestinal Organoids Using Lentiviral Vectors
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2026
Source: Van Lidth de Jeude, J. F., et al. Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (2015)This video describes the lentiviral transduction of mouse intestinal organoids from a crypt-like structure containing suspension. Using a basement membrane matrix as a scaffold and growth factor–supplemented media, lentiviral vectors are introduced to enable stable gene...
06:03
Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
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Cited by 2 •
2018
Mouse fertilized eggs and early stage embryos are protected by the zona pellucida, a glycoprotein matrix that forms a barrier against gene delivery. This article describes a protocol for perforating the zona with a laser to transduce embryonic cells with lentiviral vectors and to create transgenic mice.
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
FAQs
The process does not depend exclusively on cell division to distribute introduced genetic material. After entry, the vector releases its RNA genome, which is reverse-transcribed into DNA and integrated into the host genome. This integration provides a basis for stable expression across experimental cell types, including populations that are not actively dividing, broadening its usefulness in biological studies.
Engineered vectors are derived from lentiviruses but designed without the functions needed for replication. Their purpose is therefore to deliver a transgene rather than propagate as a replicating virus. This design is central to using the system as a gene-delivery tool, while experimental planning still requires attention to delivery performance, transgene regulation, and possible insertion-related effects.
Integration places the transgene within the host genome, which can support long-term expression rather than only transient production of the introduced genetic material. However, integration also makes genomic context relevant to interpretation. Researchers must consider whether the transgene remains appropriately regulated and evaluate potential insertion-related effects when analyzing results or planning therapeutic applications.
Three central considerations are delivery efficiency, transgene regulation, and insertion-related effects. Delivery efficiency indicates how effectively target cells receive the genetic material, while regulation concerns whether expression behaves as intended over time. Because the transgene can integrate into the host genome, assessing possible insertion-related consequences is also important for reliable biological interpretation.
A study typically begins by selecting target cells and a transgene appropriate to the biological question. Researchers then apply the engineered vector and assess whether genetic material was delivered, whether expression is maintained, and whether regulation is suitable for the experiment. These evaluations connect the delivery step to downstream gene-function studies, disease models, or cell-based applications.
The approach is useful when investigators need target cells to express introduced genetic material over an extended period. Stable expression can support experiments examining gene function or modeling disease-related biology in relevant cells. Its ability to work with dividing and nondividing cells expands the range of cellular systems that can be studied compared with approaches limited to actively proliferating populations.
In immunology and regenerative biology, the method can provide a way to introduce and maintain transgene expression in cells used for research or cell-based therapy development. Researchers can use the resulting cells to investigate biological functions or evaluate therapeutic concepts. Interpretation still depends on confirming expression behavior and considering integration-related effects in the relevant cellular context.