Lichens

Lichens are stable symbiotic associations between a fungus and a photosynthetic partner, usually a green alga or cyanobacterium, that form distinct organisms capable of living in demanding environments. The fungal partner provides structure, protection, water retention, and access to minerals, while the photosynthetic partner produces carbohydrates; cyanobacteria may also supply biologically fixed nitrogen. Lichens colonize surfaces such as rocks, bark, and soil, contributing to weathering, soil formation, nutrient cycling, and ecological succession. Because many species respond sensitively to air pollution, moisture, and environmental change, biology researchers use lichens in biodiversity studies and as indicators of ecosystem health.

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Research

JoVE Journal - Medicine

Urethroplasty with Pedicled Tunica Vaginalis for the Treatment of Long-segment Anterior Urethral Stricture Caused by Lichen Sclerosus of Glans Penis

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Cited by 1 •

2024

This article introduces a surgical method using a pedicled tunica vaginalis to treat long-segment urethral stricture resulting from lichen sclerosus. The tunica vaginalis is an excellent substitute for the urethra, leading to quick postoperative recovery for patients.

Finite Element Analysis Model for Assessing Expansion Patterns from Surgically Assisted Rapid Palatal Expansion

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2023

A set of novel finite element models of surgically assisted rapid palatal expansion (SARPE) that could perform a clinically required amount of expander activation with various angles of buccal osteotomy was created for further analysis of the expansion patterns of the hemimaxillae in all three dimensions.

Seven Steps to Stellate Cells

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Cited by 67 •

2011

Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

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Cited by 5 •

2016

In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

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Cited by 16 •

2015

Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.

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