Liver Scaffold
Liver Scaffold - Related Videos
10:18
Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
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Cited by 14 •
2016
This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.
05:20
Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
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Cited by 2 •
2021
Liver diseases are induced by many causes that promote fibrosis or cirrhosis. Transplantation is the only option for recovering health. However, given the scarcity of transplantable organs, alternatives must be explored. Our research proposes the implantation of collagen scaffolds in liver tissue from an animal model.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
02:08
Transduction of a Humanized Rat Liver Using Adeno-associated Virus Vectors
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2026
Source: Hiller, T., et al. Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2. J. Vis. Exp., (2016)This video demonstrates adeno-associated virus-mediated gene delivery in a humanized rat liver scaffold. The vector delivers a transgene encoding a fluorescent reporter and gene-silencing RNA into liver cells via receptor-mediated endocytosis. This model supports efficient transduction in a perfused 3D liver tissue system.
FAQs
The preserved extracellular matrix provides both physical organization and biochemical cues. Its native three-dimensional architecture can guide cell attachment and help organize repopulated cells within the scaffold. These features are important because the framework does more than provide support: it contributes signals that influence cell behavior and may help maintain functions relevant to engineered liver tissue.
Decellularization removes the donor tissue’s cellular components while retaining the extracellular matrix and native architecture. This creates a framework that can later be repopulated with selected liver-related cells. The central balance is preservation of structural and biochemical information without the original cellular material, allowing researchers to study how new cells interact with a liver-derived environment.
Hepatocytes and endothelial cells provide complementary cellular components for rebuilding liver-related tissue. Hepatocytes are relevant to modeling liver function, while endothelial cells are particularly important when researchers investigate vascularization. Combining cell types can therefore address both functional and structural needs, although maintaining appropriate organization and long-term performance remains challenging.
A scaffold may support initial cell organization without guaranteeing that the repopulated tissue remains functional over time. Researchers must therefore consider whether cells can maintain liver-related activity and whether vascularization can be established or sustained. These issues directly affect the feasibility of engineered tissue for advanced applications, including potential transplantation.
A typical workflow begins with donor liver tissue, followed by decellularization to remove its cellular components while preserving the extracellular matrix and native architecture. Researchers then repopulate the resulting framework with hepatocytes, endothelial cells, or other relevant cell types. The outcome depends on how effectively the new cells attach, organize, and maintain function within the preserved structure.
Researchers can repopulate a Liver Scaffold with hepatocytes, endothelial cells, or other relevant cell types selected for the intended model or repair strategy. Hepatocytes support investigations of liver function, whereas endothelial cells address aspects connected with vascularization. The choice of cells allows the scaffold to be adapted for disease studies, drug testing, or regenerative research.
Repopulated liver scaffolds can provide three-dimensional systems for modeling liver function and studying disease within a framework that retains native architecture and extracellular-matrix cues. They may also support drug-testing research by offering an engineered tissue context. These applications help investigators examine cell behavior and tissue responses in a more organized setting than isolated cells alone.
Before transplantation becomes feasible, researchers must address the scaffold’s ability to support vascularization and preserve cell function over the long term. The repopulated tissue must remain organized and biologically active rather than functioning only temporarily after preparation. These requirements make transplantation a longer-term goal of scaffold research rather than an automatic result of decellularization and repopulation.