Lung Embedding
Lung Embedding - Related Videos
07:42
A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
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Cited by 4 •
2014
Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.
03:39
Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)
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2023
This protocol describes the preparation of formalin-fixed paraffin-embedded tumor samples which are used to preserve tissue and cytology samples from non-small cell lung cancer...
05:00
Multiplex Immunohistochemistry Staining for Paraffin-embedded Lung Cancer Tissue
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Cited by 2 •
2023
This experimental protocol describes and optimizes a multiplex immunohistochemistry (IHC) staining method, mainly by optimizing single-channel antibody incubation conditions and adjusting the settings of antibodies and channels to solve the problems of autofluorescence and channel crosstalk in lung cancer tissues of clinical origin.
07:17
Automated Vibratome Sectioning of Agarose-Embedded Lung Tissue for Multiplex Fluorescence Imaging
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Cited by 5 •
2023
We have developed a tissue-processing technique utilizing a vibratome and agarose-embedded lung tissue to generate lung sections, thereby permitting the acquisition of high-resolution images of lung architecture. We employed immunofluorescence staining to observe spatial protein expression using specific lung structural markers.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
FAQs
A supporting medium, such as paraffin, stabilizes the fixed lung by forming a firm block around its structures. This mechanical support allows thin sections to be cut while reducing disruption of delicate airways, alveoli, blood vessels, and lesion boundaries. The resulting sections provide a more reliable basis for staining and microscopic comparison across different regions of the specimen.
Orientation determines how anatomical structures appear in the final sections. Positioning the lung appropriately within the mold can help preserve meaningful relationships among airways, alveoli, blood vessels, and lesions, making their boundaries easier to examine. Consistent orientation also supports comparisons between samples and improves interpretation of tissue architecture in relation to respiratory function or disease.
Delicate airways and alveoli are particularly vulnerable to distortion when tissue support is insufficient, while blood vessels and lesion boundaries may become harder to distinguish. Because these features are central to evaluating lung architecture, poor preservation can limit microscopic assessment of inflammation, fibrosis, tumors, or treatment-related changes. Careful embedding therefore affects both structural detail and interpretation.
The preparation proceeds from fixation to dehydration, clearing, infiltration with a supporting medium, and placement in a mold for block formation. Each stage prepares the specimen for the next: fixation preserves tissue, dehydration and clearing condition it for infiltration, and the supporting medium creates a firm block. Thin sections can then be cut, stained, and imaged.
Embedded lung tissue enables microscopic evaluation of changes associated with inflammation, fibrosis, tumors, and treatment-related effects. Researchers can examine these findings alongside preserved airways, alveoli, blood vessels, and lesion boundaries, allowing abnormal patterns to be related to overall tissue architecture. This makes the preparation useful for comparing diseased and otherwise normal lung structure.
Microscopic sections reveal how lung tissue architecture is organized and how that organization changes during disease or after treatment. Observations of airways, alveoli, blood vessels, and lesions can be correlated with respiratory function and disease mechanisms. In biology, this structural context helps connect visible tissue changes with broader questions about lung health and pathology.