Marginal Liver Grafts
Marginal Liver Grafts - Related Videos
09:29
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
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Cited by 31 •
2014
Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.
03:42
Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
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2025
In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.
06:53
Selective Harvesting of Marginating-hepatic Leukocytes
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Cited by 1 •
2016
Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases.
07:06
Selective Harvesting of Marginating-pulmonary Leukocytes
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Cited by 1 •
2016
Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
FAQs
Reduced blood flow during preservation can leave a vulnerable liver less able to tolerate the sudden return of circulation. When blood flow is restored, ischemia-reperfusion injury may contribute to poor early graft function. This mechanism explains why prolonged ischemia is an important risk factor and why preservation strategies are central to improving outcomes with higher-risk organs.
Older donor age and steatosis are characteristics associated with reduced hepatic resilience. A graft with less functional reserve may be more susceptible to injury during preservation and after circulation resumes. Assessing these features helps researchers and transplant teams estimate risk before transplantation and consider whether additional preservation or selection measures are warranted.
Donation after circulatory death can increase concern about graft vulnerability because the liver may experience conditions that challenge its resilience before transplantation. In combination with factors such as donor age, steatosis, or prolonged ischemia, it contributes to a more complex risk profile. Evaluating these characteristics together supports more informed graft viability assessment.
Machine perfusion provides an emerging preservation approach for assessing and potentially improving the viability of Marginal Liver Grafts. Rather than relying only on donor characteristics, researchers can study the graft under controlled perfusion conditions before transplantation. This may help distinguish organs that remain suitable from those less likely to function adequately after implantation.
Decision-making combines donor assessment, recipient selection, and preservation considerations. Donor age, steatosis, ischemia duration, and donation after circulatory death contribute to the graft risk profile, while the intended recipient must also be considered. Together, these evaluations help determine whether the potential expansion of the organ supply justifies the anticipated risk of poor function.
Preservation strategies aim to reduce vulnerability while the graft is outside the body and to support later assessment of its condition. The source material identifies emerging machine perfusion methods as an approach that can evaluate and potentially improve viability. These strategies are especially relevant when donor characteristics or ischemia raise concern about graft resilience.
Studying these grafts can support safer use of organs that might otherwise remain underutilized, helping address the limited organ supply for patients with advanced liver disease. In biology and transplant research, they also provide a context for investigating hepatic regeneration and graft dysfunction, linking practical transplantation decisions with broader studies of liver resilience and recovery.
Marginal graft research connects preservation and transplantation outcomes with the liver’s capacity to recover after stress. By examining graft dysfunction, viability, and responses to preservation or perfusion, investigators can study how hepatic tissue withstands injury and potentially regenerates. This biological context may guide approaches for using higher-risk organs more safely in transplantation.