Mesenchymal Induction
Mesenchymal Induction - Related Videos
10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
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Cited by 16 •
2013
A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
11:33
Inductance
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN This experiment will use inductive coils to demonstrate the concept of inductor and inductance. Magnetic induction will be demonstrated using a rod magnet inserted into or extracted away from the core of a coil to induce a transient electromotive force (emf) voltage in the coil, measured by a voltmeter. This experiment will also demonstrate the mutual inductance between...
06:02
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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Cited by 16 •
2016
Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.
07:23
Electroporation of Craniofacial Mesenchyme
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2011
Craniofacial cartilages develop in close contact with other tissues and are difficult to manipulate in live animals. We are using electroporation to deliver molecular tools during growth of the craniofacial skeleton while bypassing early embryonic effects. This approach will allow us to efficiently test candidate molecules in vivo.
FAQs
These signals influence mesenchymal induction by changing gene expression programs that control adhesion, polarity, movement, and tissue remodeling. Their activity can shift cells away from tightly organized epithelial behavior and toward characteristics needed for migration and connective-tissue contribution. Comparing these signaling inputs helps researchers examine how different developmental or repair contexts produce related cellular changes.
Key indicators include reduced cell-cell adhesion, loss or disruption of epithelial polarity, and reorganization of the cytoskeleton. Together, these changes alter how cells maintain their shape, interact with neighbors, and move through tissue. Researchers can evaluate these features alongside gene-expression changes to connect molecular signaling with the resulting cellular behavior.
Cytoskeletal reorganization provides a structural basis for altered cell movement and remodeling. As cells change their internal architecture, they can reduce dependence on stable epithelial organization and acquire behavior more compatible with migration through developing or repairing tissue. This mechanism links changes in gene expression and adhesion to the physical actions required during tissue formation.
A study can examine the process by exposing cells or tissues to relevant signals, then assessing gene expression, cell-cell adhesion, epithelial polarity, cytoskeletal organization, and movement. These observations provide complementary evidence rather than relying on a single marker. The approach is useful for comparing developmental, repair, stem-cell, or disease-associated settings.
During embryonic development, the process supports tissue formation by enabling cells to move, remodel their surroundings, and contribute to connective tissues. In wound healing, similar cellular changes help reorganize damaged regions. Studying both settings can reveal how a shared biological program supports normal formation and repair while producing different tissue-level outcomes.
The same cellular changes that support movement and remodeling can also help researchers investigate abnormal tissue behavior. In fibrosis, mesenchymal induction provides a framework for studying remodeling associated with excessive scar-like tissue formation. In tumors, it helps researchers examine invasive behavior. These disease contexts connect developmental biology with mechanisms relevant to pathological tissue change.
Mesenchymal induction can be used as part of strategies for directing pluripotent or adult stem cells toward mesenchymal characteristics and connective-tissue contributions. Researchers study the relevant signals and cellular responses to guide differentiation. This makes the process relevant to engineered-tissue approaches, where controlled cell behavior is needed to generate organized biological tissue.