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Electroporation of Craniofacial Mesenchyme
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Electroporation of Craniofacial Mesenchyme
DOI:

07:23 min

November 28, 2011

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Chapters

  • 00:05Title
  • 01:19Preparation of Equipment
  • 03:38Micropipette Setup
  • 04:10Preparation of Tadpole
  • 05:16Electroporation
  • 06:13Electroporation Results
  • 07:00Conclusion

Summary

Automatic Translation

Craniofacial cartilages develop in close contact with other tissues and are difficult to manipulate in live animals. We are using electroporation to deliver molecular tools during growth of the craniofacial skeleton while bypassing early embryonic effects. This approach will allow us to efficiently test candidate molecules in vivo.

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