Microforge Rig
Microforge Rig - Related Videos
12:43
Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
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Cited by 12 •
2014
Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.
06:57
Fabrication and Testing of Miniature Automatic Photophoretic Trapping Rigs
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Cited by 2 •
2021
This work describes and characterizes the fabrication of miniature automatic photophoretic trapping rigs.
08:59
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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Cited by 25 •
2016
This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.
12:45
Mechanical Stimulation of Chondrocyte-agarose Hydrogels
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Cited by 29 •
2012
The biosynthesis of cartilaginous extracellular matrix by chondrocytes can be affected by application of mechanical stimuli. This method describes the technique of applying dynamic compressive strains to chondrocytes encapsulated in 3D constructs and the evaluation of induced changes in chondrocyte metabolism.
02:43
Microelectrode-Mediated FM Dye Loading and Unloading in Drosophila Larvae Nerve Cells
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2025
In this video, Drosophila larvae nerve fibers are immersed in FM dye, which binds to cell membrane lipids. Suction microelectrode and electrical pulses stimulate the endocytosis process, incorporating the FM dye into newly formed endocytic vesicles. This method, which visualizes presynaptic stages of neurotransmission, is useful for studying synaptic function and neurotransmitter release.
FAQs
Localized heating softens the selected region of a fine glass or metal tool, allowing mechanical manipulation to produce a controlled bend, constriction, bevel, or closed tip. Because the heat is applied only where modification is needed, the operator can alter tip geometry while preserving the rest of the tool. This supports precise matching between the tool and the biological task.
Magnification lets the operator observe the tool tip and its position relative to the forming process, while mechanical manipulation determines how the softened material moves. Together, these functions provide control over dimensions, alignment, and geometry. That control matters when a small difference in tip shape can affect access to, contact with, or handling of a delicate biological specimen.
The rig can combine localized heating with controlled movement to form bends, bevels, constrictions, or closed tips. Some systems also provide polishing or sealing, adding further refinement after the primary shape is created. Selecting among these modifications allows researchers to adapt the tool's dimensions and endpoint configuration to the requirements of injection, electrophysiology, manipulation, or microsurgery.
A typical workflow places the micropipette or probe in the rig, positions it under magnification, and applies localized heating while mechanically shaping the selected region. The operator then refines the geometry, using polishing or sealing when those functions are available. The finished tool can be checked for the intended bend, bevel, constriction, or closure before biological use.
Customized microtools can support microinjection, patch-clamp electrophysiology, embryo manipulation, cell handling, and microsurgical procedures. Each application may require a different combination of dimensions and tip geometry, so shaping the tool for the task can improve its fit to the specimen or experimental setup. The instrument is therefore useful across both cellular and developmental biology workflows.
More consistent tool dimensions and tip geometries can improve experimental control and reproducibility by reducing variation between tools. A better-fitting tip may also support safer manipulation of delicate specimens during procedures such as cell handling, embryo manipulation, or microsurgery. In electrophysiology and microinjection, controlled geometry helps align the tool design with the demands of the experiment.