Microtip Drug Application
Microtip Drug Application - Related Videos
09:03
Manufacture and Drug Delivery Applications of Silk Nanoparticles
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Cited by 49 •
2016
Nanoparticles are emerging as promising drug delivery systems for a broad range of indications. Here, we describe a simple yet powerful method to manufacture silk nanoparticles using reverse engineered Bombyx mori silk. These silk nanoparticles can be readily loaded with a therapeutic payload and subsequently explored for drug delivery applications.
13:29
Biodistribution of Nano-drug Carriers: Applications of SEM
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Nanoparticles have been increasingly used research towards targeted drug delivery and controlled drug release. While most of these particles have been developed as polymeric or liposomal particles because of their biocompatibility, there is a trend in current research toward the use of metallic and magnetic nanoparticles. These metallic nanoparticles...
13:17
The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
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Cited by 48 •
2015
Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.
10:04
Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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Cited by 23 •
2012
In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.
07:23
An All-Human Hepatic Culture System for Drug Development Applications
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Cited by 6 •
2023
Recent technical advances enabled the scaled production of an in vitro platform for drug metabolism and toxicity applications. An all-human 2D+ hepatic system (TV2D+) provides physiologically relevant results using traditional two-dimensional culture methods. This protocol will support end-users in the setup, maintenance, and application of the system.
FAQs
Spatial control comes from concentrating a small compound quantity at a defined point of contact rather than exposing a broad region. This localized placement helps researchers associate an observed cellular or tissue response with the treated site, while limiting direct treatment of neighboring areas. The approach is therefore useful when the experiment requires focused pharmacological manipulation.
Restricting exposure helps separate direct drug action from effects produced by broader treatment. Cells or tissues outside the targeted area experience less intentional compound exposure, making comparisons between treated and nearby untreated regions more informative. This distinction can clarify whether a response originates at the application site or reflects secondary consequences of widespread delivery.
Because the technique places a small amount of compound at a selected biological site, researchers can examine how responses vary with the amount applied at that location. Such comparisons may reveal whether cellular or tissue effects change as exposure increases or decreases. This supports focused analysis of pharmacological activity without requiring treatment of the entire experimental system.
A basic workflow is to identify the biological target, position the fine tip at the intended point of contact, and place a small amount of the compound onto or into that site. Researchers can then observe the local cellular or tissue response and compare it with areas that did not receive the focused application.
Researchers may choose it when they need to manipulate a particular cell, tissue region, or physiological site while minimizing treatment elsewhere. It is especially relevant for studies of localized pharmacological effects, cell responses, and tissue physiology. The focused format can also reduce reagent use compared with broader exposure, which is valuable in controlled experiments.
This approach can provide information about how a defined biological location responds to a compound and whether the response depends on the applied amount. By focusing exposure at the point of contact, researchers can evaluate local drug activity, compare treated and surrounding regions, and better distinguish direct effects from responses associated with more extensive treatment.