An All-Human Hepatic Culture System for Drug Development Applications

Justin J. Odanga; Elena Gianulis; Lindsey Whaley; Edward L. LeCluyse; Sharon Presnell; Jessica R. Weaver

doi:10.3791/65992

Journal

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Biology

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An All-Human Hepatic Culture System for Drug Development Applications

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

An All-Human Hepatic Culture System for Drug Development Applications

1,206 Views

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07:23 min

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October 20, 2023

DOI:

10.3791/65992-v

DOI:

10.3791/65992-v

•

07:23 min

October 20, 2023

•

10 Views

Justin J. Odanga¹, Elena Gianulis², Lindsey Whaley³, Edward L. LeCluyse⁴, Sharon Presnell¹, Jessica R. Weaver¹

¹Institute of Regenerative Medicine,LifeNet Health, ²Biologics Division,LifeNet Health, ³LifeSciences Division,LifeNet Health, ⁴Research and Development,LifeSciences, LifeNet Health

Transcript

TruVivo was developed to fill the need for convenient yet robust in vitro platform that maintains phenotypically stable and functional primary human hepatocytes. Because of these qualities, the system serves a basic platform for answering a wide range of questions pertaining to preclinical drug development. One challenge is the need to create a multicellular system that has the biological complexity required to answer today’s questions, yet is still easy to use.

Current systems often fail to maintain hepatocyte morphology and function long-term or present technical challenges with establishing routine, reproducible results. With TruVivo, users can still rely on the simplicity and flexibility of traditional 2D cell culture techniques. Additionally, there is no special equipment required, and the system is amenable to traditional analytical methods.

With TruVivo, we are now able to predict metabolic clearance, major metabolites, key pathways of elimination, and other pharmacokinetic properties of compounds more accurately with long half-lives prior to preclinical evaluation.

Summary

Recent technical advances enabled the scaled production of an in vitro platform for drug metabolism and toxicity applications. An all-human 2D+ hepatic system (TV2D+) provides physiologically relevant results using traditional two-dimensional culture methods. This protocol will support end-users in the setup, maintenance, and application of the system.