Microvessel Formation
Microvessel Formation - Related Videos
08:27
Endothelial Cell Culture from Rat Brain Microvessels: A Procedure to Isolate and Culture Endothelial Cells from Microvessels of Rat Brain
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2023
This video presents the isolation of microvessels from a rat brain using a combination of density gradient centrifugation and enzymatic digestion mehods. The isolated microvessels can be cultured in a culture flask pre-coated with collagen and fibronectin matrix to obtain endothelial cell colonies.
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
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Cited by 3 •
2015
The modified Landis technique enables paired measurement of the hydraulic conductivity of individual microvessels in the mesentery of normal and genetically modified rats under control and test conditions using microperfusion techniques. It provides a convenient method to evaluate mechanisms that regulate microvessel permeability and transvascular exchange under physiological conditions.
13:05
Micropatterning and Assembly of 3D Microvessels
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Cited by 12 •
2016
This manuscript presents an injection molding method to engineer microvessels that recapitulate physiological properties of endothelium. The microfluidic-based process creates patent 3D vascular networks with tailorable conditions, such as flow, cellular composition, geometry, and biochemical gradients. The fabrication process and examples of potential applications are described.
07:22
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
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Cited by 5 •
2014
The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.
![Development and Characterization of <em>In Vitro</em> Microvessel Network and Quantitative Measurements of Endothelial [Ca<sup>2+</sup>]<sub>i </sub>and Nitric Oxide Production](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F54014.jpg%3Fv%3D2026-09-01%252006%253A38%253A51&w=750&q=75)
09:39
Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
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Cited by 7 •
2016
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.
FAQs
Microvessel formation proceeds through a coordinated endothelial-cell response rather than a single event. Cells respond to vascular endothelial growth factor, break down the surrounding extracellular matrix, migrate into the remodeled space, proliferate, and then organize into tubes with an internal lumen. This sequence converts a local signal into new vascular structures capable of joining an existing network.
The extracellular matrix provides the surrounding framework that endothelial cells must modify before they can move and organize. Its degradation opens a path for migration, while subsequent cell organization supports tube assembly. This makes matrix remodeling a functional step linking the initial growth signal to the structural changes required for developing microvessels.
A lumen gives the developing tube an internal passage, while connection to an existing vascular network links the new structure to tissue perfusion. Together, these features distinguish organized vessel development from isolated endothelial-cell growth. They help explain how the process can support oxygen and nutrient delivery and waste exchange within tissue.
The underlying vessel-building process supports normal development, wound healing, and tissue repair, where new circulation helps meet tissue needs. The same capacity can become abnormal in cancer and other diseases, making regulation and context important. Biology studies therefore examine not only how vessels form, but also when their growth is beneficial or pathological.
A useful sequence follows the major transitions described for angiogenesis: vascular endothelial growth factor response, extracellular-matrix degradation, endothelial migration, cell proliferation, tube organization, lumen formation, and network connection. Separating these stages helps relate molecular or cellular changes to structural outcomes and clarifies where a biological intervention may influence vessel development.
New microvessels are relevant to regenerative medicine because tissue repair depends on restoring perfusion and exchange. Engineered tissue models use the process to investigate vascular organization, while drug-screening studies can examine effects on vessel growth. These applications connect basic vascular biology with tissue design and disease-focused research, including studies of abnormal growth in cancer.