Mouse Model Systems
Mouse Model Systems - Related Videos
09:52
A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
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Cited by 15 •
2014
Animal models have proven to be invaluable tools in defining host and pathogen specific mechanisms that contribute to the development of chronic inflammation. Here we describe a mouse model of oral infection with the human pathogen Porphyromonas gingivalis and detail methodologies to assess the progression of inflammation at local and systemic sites.
03:50
Systemic Pilocarpine Treatment: Mouse Model of Temporal Lobe Epilepsy
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2023
This video presents the procedure for inducing status epilepticus (SE) in mice by systemic administration of pilocarpine to model temporal lobe epilepsy.
03:52
Quantification of Bacterial Load in Mouse Spleen and Liver After Systemic Infection
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2026
Source: Ahn, J. J., et al. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (2016)The video demonstrates bacterial load measurement in spleen and liver tissues isolated from mice infected with an intracellular bacterial pathogen. Bacteria, accumulated within tissue-resident phagocytes during systemic infection, are released using detergent-based lysis and mechanical disruption. Serial dilutions of the homogenates are plated on...
18:57
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Cited by 58 •
2013
Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.
01:14
Pharmacodynamic Models: Link Model and Systems Pharmacodynamic Model
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2026
The link model is a fundamental pharmacokinetic-pharmacodynamic (PK–PD) approach to account for delayed drug responses when the observed effect does not immediately correlate with the drug's plasma concentration peak. This delay is mathematically addressed by introducing an effect compartment concentration, Ce, which is kinetically linked to the plasma concentration, Cp, via a first-order rate constant, ke0. The linkage allows for a more accurate prediction of drug effects over time. A higher...
FAQs
Genetic modifications allow researchers to associate a particular gene or genetic change with measurable physiological or disease-related outcomes. When the altered mice are studied through controlled breeding, phenotyping, and molecular analysis, investigators can examine whether the change affects a biological pathway or observable trait. This supports causal interpretation rather than relying only on correlations between genes and disease.
Genetic background provides the biological context in which a mutation, physiological alteration, or disease process appears. Inbred strains offer a more controlled context, while genetically modified and disease-induced strains address different experimental questions. Comparing outcomes across appropriately selected models can help distinguish effects linked to the target change from effects associated with the broader strain context.
Controlled environmental conditions help researchers interpret phenotypic differences more reliably. Factors managed during the study can influence physiology and therefore affect how genetic or disease-related changes appear. Keeping these conditions consistent supports comparisons among experimental groups and makes it easier to connect observed outcomes with the biological variable under investigation rather than with uncontrolled surroundings.
Phenotyping records observable outcomes, whereas molecular analysis examines biological changes associated with those outcomes. Used together, they connect a gene, pathway, or physiological alteration with both its measurable effects and underlying molecular features. This combined evidence can help identify disease mechanisms, reveal biomarkers, and clarify how a model reflects the process being studied.
A study begins by selecting an appropriate inbred, genetically modified, or disease-induced strain for the biological question. Researchers then apply controlled breeding and environmental conditions, measure phenotypes, and perform molecular analysis. Interpreting these results requires relating the specific genetic or physiological change to observed outcomes and considering how well the model represents the intended disease or process.
Mouse model systems support investigations across development, immunity, cancer, metabolism, neuroscience, and infectious disease. Their value differs by question: a study may examine gene function, disease mechanisms, physiological responses, or treatment effects. This broad applicability allows researchers to connect controlled biological changes with outcomes in multiple areas of biology rather than limiting the approach to one disease category.
These models can support evaluation of potential therapies before clinical research by showing how a treatment performs in a controlled biological context. Conserved biological pathways may make the findings informative, but mouse results do not automatically reproduce human biology. Researchers must therefore consider the model's strengths and limits when interpreting treatment responses, biomarkers, and disease mechanisms.