Nadp Reductase
Nadp Reductase - Related Videos
01:27
Photosystem I
0 Views •
2019
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor. Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
08:45
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
0 Views •
Cited by 84 •
2011
A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.
10:31
Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation
0 Views •
Cited by 41 •
2010
In this video, techniques for multicolor confocal time-lapse imaging and targeted cell ablation are provided. Time-lapse imaging is used to monitor the behavior of multiple cell types of interest in vivo. Targeted cell ablation facilitates the study neural circuit function and cell-specific neuronal regeneration paradigms.
02:45
Bacterial Growth on a Microfluidic Chip to Study Antibiotic Drug Resistance
0 Views •
2025
Source: Liu, P. C., et al. Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection. J. Vis. Exp. (2016)This video demonstrates the use of a microfluidic chip to visualize and compare the growth and morphology of wild-type and trimethoprim-resistant E. coli strains under trimethoprim antibiotic stress.
03:01
alamarBlue Assay: A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells
0 Views •
2025
This video describes an in vitro fluorometric assay to assess the effects of artificial tear formulations on the cellular metabolic activity of corneal epithelial cells using resazurin-based Alamar blue solution. A non-toxic formulation maintains the cells in a healthy, metabolically active state, facilitating the reduction of resazurin into a highly fluorescent resorufin.
FAQs
The FAD cofactor provides the enzyme’s electron-transfer center. NADP reductase uses this flavin-associated component to receive electrons from reduced ferredoxin and pass them to NADP+. Because FAD mediates the transfer rather than serving as the final product, it connects the incoming electrons with formation of NADPH. This arrangement explains why the enzyme is classified as a flavoprotein.
Reduced ferredoxin serves as the immediate electron source for the reaction. In photosynthetic organisms, light-dependent reactions generate this reduced carrier, allowing NADP reductase to capture reducing power produced during light capture. The enzyme therefore links the flow of photosynthetic electrons with the later production of NADPH.
The chloroplast stroma is the site where the enzyme transfers electrons to NADP+. This location places NADPH production on the side of the photosynthetic system that supports carbon fixation. It also connects light-dependent electron generation with the chemical reactions that consume reducing power for carbon fixation and other biosynthetic reactions.
NADPH matters because it supplies electrons for carbon fixation and other biosynthetic reactions. Its formation converts reducing power generated during photosynthetic electron flow into a form that downstream chemistry can use. Consequently, NADP reductase helps connect energy captured from light with synthesis and cellular redox balance.
Examining NADP reductase can reveal how photosynthetic electron flow is connected to cellular redox balance. Because the enzyme receives electrons associated with light-dependent reactions and directs them toward NADPH production, it provides a focused point for studying the transition from light capture to chemical energy storage. This perspective is relevant to understanding photosynthetic biology as an integrated system.
Knowledge of NADP reductase can support efforts to engineer organisms or enzymes that produce valuable chemicals. The rationale is that the enzyme participates in a key route for generating NADPH, whose reducing power drives carbon fixation and other biosynthetic reactions. Altering this photosynthetic electron-to-biosynthesis connection could therefore guide such engineering.