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Chapters
- 00:00Title
- 00:47Introduction
- 01:01Selecting and Preparing Embryos/Larvae for Live Imaging
- 03:02“Multicolor” in vivo Confocal Imaging
- 05:05Spectral Separation Image Processing Using ImageJ Freeware
- 08:09Representative Images of Neural Loss and Regeneration
- 10:12Conclusion
In this video, techniques for multicolor confocal time-lapse imaging and targeted cell ablation are provided. Time-lapse imaging is used to monitor the behavior of multiple cell types of interest in vivo. Targeted cell ablation facilitates the study neural circuit function and cell-specific neuronal regeneration paradigms.
Tags
Multicolor Time-lapse ImagingTransgenic ZebrafishRetinal Stem CellsNeuronal Cell AblationHigh-resolution ImagingLiving Zebrafish LarvaeFluorescent ReportersCellular InteractionsExperimental ManipulationsSerial ImagingFluorophoresHypopigmented Mutant LinesMembrane Targeted Fluorescent ReportersMorphological DetailCellular DetailsChemically-induced AblationNitroreductase Mediated Ablation
