Nanoparticle Screening
Nanoparticle Screening - Related Videos
09:51
Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
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Cited by 6 •
2017
Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.
03:53
An Assay to Screen Bioactive Nanoparticles for Toll-Like Receptor Signaling Inhibition
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2025
This video demonstrates a reporter cell-based assay for screening potential bioactive nanoparticles that inhibit Toll-like receptor (TLR) signaling. The assay involves introducing lipopolysaccharides (LPS) mixed with peptide-gold nanoparticle hybrids to reporter macrophages expressing the reporter proteins — secreted embryonic alkaline phosphatase (SEAP) and luciferase. The reduction of LPS-induced TLR signal mediated by the hybrids is determined by measuring the reporter signals.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
Biological responses can vary with particle size, shape, surface charge, composition, and functionalization. Screening compares these properties to determine how they influence cellular uptake, viability, toxicity, and interactions with biomolecules. Examining the properties alongside measured responses helps connect a material’s physical and chemical characteristics with its biological performance and identifies formulations that warrant further study.
No single property necessarily explains every biological outcome. Comparing size, shape, charge, composition, and surface functionalization creates a broader view of how formulations behave in biological systems. This multi-factor approach can reveal desirable activity, harmful effects, or variability associated with particular formulations, supporting more informed selection of materials for biomedical research.
These assays address different aspects of nanoparticle behavior. Cellular uptake indicates whether particles enter cells, whereas viability reflects whether cells remain alive under the tested conditions. Toxicity measures harmful effects, and biomolecular-interaction assays examine associations with biological molecules. Considering the results together helps distinguish useful biological activity from effects that may limit safety or application.
A screening workflow begins by selecting nanoparticle formulations that differ in relevant physical or chemical properties, then evaluating them with biological assays. Measurements can include cellular uptake, viability, toxicity, and interactions with biomolecules. Comparing the resulting profiles helps researchers identify formulations with desirable activity, detect harmful effects, and recognize sources of variability between materials.
In drug-delivery research, screening helps compare formulations during candidate optimization. Researchers can examine how differences in size, shape, surface charge, composition, or functionalization relate to cellular uptake, viability, toxicity, and interactions with biomolecules. The resulting comparisons support selection of formulations with desirable activity while drawing attention to harmful effects that could affect biomedical use.
For diagnostic and imaging agents, screening provides a way to evaluate biological behavior alongside the material design. Assays can show cellular uptake, effects on viability, toxicity, and interactions with biomolecules. These results help compare candidate formulations, identify undesirable biological effects, and guide development of agents for biomedical research while keeping safety-related behavior visible.