P62 Colocalization
P62 Colocalization - Related Videos
11:39
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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Cited by 94 •
2017
Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.
03:13
Immunostaining to Visualize Viral Antigen and Host Protein Colocalization in the Insect Gut
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2026
Source: Zhang, L., et al. Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts. J. Vis. Exp. (2021)The video demonstrates the preparation of gut tissue from a plant virus–infected insect vector for fluorescence imaging. Fixed guts were washed, permeabilized, and incubated with two fluorescent antibodies in a blocking solution—one targeting a viral antigen in vesicles, the other a vesicle-associated membrane protein. The tissue was counterstained for actin...
12:58
Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
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Cited by 3 •
2019
We describe a comprehensive and practical protocol for fluorescence recovery after photobleaching experiments with live cells. Although the protocol was used to measure the mobility of yellow fluorescent protein-tagged p62 in aggresome-like induced structures, it can be applied to a variety of microscopy systems and fluorescent proteins.
08:44
Activating Autophagy by Aerobic Exercise in Mice
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Cited by 37 •
2017
Autophagy activation is beneficial in the prevention of a number of diseases. One of the physiological approaches to induce autophagy in vivo is physical exercise. Here we show how to activate autophagy by aerobic exercise and measure autophagy levels in mice.
07:48
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
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Cited by 1 •
2015
Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.
FAQs
p62/SQSTM1 provides a protein signal linked to selective autophagy and protein aggregation. When its fluorescence overlaps a marker for an autophagosomal, lysosomal, or disease-associated structure, the result helps relate p62 distribution to cargo processing, degradation, aggregation, or cellular pathology. This makes the spatial measurement relevant to intracellular organization rather than simply a signal-counting exercise.
The second marker determines which biological association the analysis can address. An autophagosomal marker focuses the measurement on p62 in relation to autophagy-related structures, while a lysosomal marker directs attention toward structures associated with degradation. Disease-associated markers allow examination of p62 alongside cellular features linked with disease. Marker selection therefore shapes the biological interpretation of overlapping signals.
Comparable acquisition conditions make differences in overlapping fluorescence more suitable for interpretation. If imaging conditions vary substantially between samples, changes in signal overlap may be difficult to relate to altered intracellular organization or experimental treatment. Consistent conditions support the subsequent quantification of p62 and the second target, helping researchers evaluate biological differences rather than inconsistent image acquisition.
Researchers first label p62 and a second cellular target, then acquire fluorescence images under comparable conditions. Colocalization analysis is applied to the resulting signals to quantify their spatial overlap. The measured overlap can then be considered alongside the selected marker, allowing the study to assess p62 association with autophagosomal, lysosomal, or disease-associated structures in the biological sample.
A basic workflow requires a biological sample, labeling for p62, labeling for a second cellular marker, fluorescence microscopy, and an analysis procedure for overlapping signals. The two targets must be imaged under comparable conditions so their spatial relationship can be evaluated. This combination connects molecular labeling, image acquisition, and quantitative analysis in a single experimental approach.
This approach is useful when researchers want to determine how genetic or pharmacological interventions alter intracellular organization. Comparing p62 overlap with selected markers can show whether an intervention changes its association with autophagosomal, lysosomal, or disease-associated structures. Such comparisons support investigations of altered cargo processing, degradation, proteostasis, or cellular stress within biological samples.
p62 colocalization provides spatial context for studying processes that maintain protein and cellular organization. Because p62 participates in selective autophagy and may accumulate in protein aggregates, its overlap with relevant markers can help investigate cargo handling, degradation, and stress-related changes. In biology research, these measurements support broader analyses of autophagy, proteostasis, and disease-associated intracellular organization.