Nerve Growth Factor
Nerve Growth Factor - Related Videos
03:06
Encapsulating Nerve Growth Factor in High-Density Lipoprotein-Mimicking Nanoparticles
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2025
Source: Zhu, J., et. al., Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation. J. Vis. Exp. (2017)This video demonstrates the stepwise assembly of NGF-loaded HDL-mimicking nanoparticles using protamine-assisted encapsulation and lipid-based self-assembly for potential neurotrophic delivery applications.
08:24
Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
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Cited by 4 •
2017
Simple homogenization was used to prepare novel, high-density, lipoprotein-mimicking nanoparticles to encapsulate nerve growth factor. Challenges, detailed protocols for nanoparticle preparation, in vitro characterization, and in vivo studies are described in this article.
01:44
Sustained Delivery of Nerve Growth Factor Using a Porous Silicon Film in Rat Pheochromocytoma 12 Cells
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2025
Source: Rosenberg, M., et al. Designing Porous Silicon Films as Carriers of Nerve Growth Factor. J. Vis. Exp. (2019).This video demonstrates the use of a degradable nanostructured porous silicon (PSi) film for the sustained release of nerve growth factor (NGF) to support PC12 cell differentiation. NGF is adsorbed onto the film’s pores through electrostatic interactions and is gradually released into the media by diffusion and scaffold degradation. This controlled delivery promotes PC12 cell...
10:12
Designing Porous Silicon Films as Carriers of Nerve Growth Factor
0 Views •
Cited by 3 •
2019
Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.
09:29
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
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Cited by 10 •
2014
This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.
FAQs
Activation of TrkA by NGF initiates intracellular signaling pathways that influence gene expression. These changes help regulate axon growth and maintain neuronal function. The receptor therefore links an extracellular signal with longer-term changes inside a responsive neuron, rather than merely producing a brief binding event.
NGF can also interact with the p75 neurotrophin receptor, which modifies cellular responses to the signal. This means NGF effects cannot be understood from ligand binding alone; researchers must consider the receptor systems present on responsive cells. That receptor context helps explain why one signaling protein may contribute to different neuronal outcomes.
Gene expression gives NGF signaling a longer-term cellular dimension. After receptor activation, changes in gene expression can influence processes associated with axon growth and neuronal maintenance. This helps researchers connect an initial extracellular signal with sustained changes in neuron behavior, which is important when studying development, function, or responses to injury.
NGF's influence on sensory neuron signaling connects it with research on pain and inflammation. This relationship broadens its biological significance beyond neuronal development and maintenance, allowing investigators to examine how sensory neurons participate in these responses. It also makes NGF relevant when studying changes in nervous system signaling associated with injury-related conditions.
NGF research can connect several questions: how neurons establish connections, how they respond to injury, and how signaling supports neuronal survival and function. These investigations place NGF within broader studies of nervous system development, nerve repair, neurodegenerative disease, and possible neuroprotective therapies, providing a framework for examining related biological processes.
Because NGF supports neuronal survival and maintenance, its signaling is relevant to research on neurodegenerative disease. By examining pathways that influence neuronal function and gene expression, investigators can explore how neurons remain functional and why protective signaling matters. This work supports investigation of potential neuroprotective therapies without implying that NGF alone prevents disease.