Ocular Structure Analysis

Ocular structure analysis is the systematic examination of the eye’s anatomy to characterize how its components support vision and maintain visual function. It assesses structures such as the cornea, iris, lens, retina, and optic nerve through anatomical observation, microscopy, or imaging, relating their form to light transmission, focusing, photoreception, and neural signaling. In biology, this analysis helps students and researchers compare normal and altered eye structures, investigate developmental and pathological changes, and interpret how tissue organization influences visual performance. These findings support research in sensory biology, ophthalmology, neuroscience, and the evaluation of ocular disease.

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Research

JoVE EoE - Immune Response

A Method to Induce Ocular Surface Inflammation in a Murine Model

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2025

This video demonstrates a method to induce ocular surface inflammation and meibomian gland dysfunction in a murine model. Stimulation of the mouse's immune system using an immunogen and later restimulation with the same immunogen promotes ocular surface inflammation, which causes plugging of meibomian gland secretion through neutrophil-produced aggNETs and leads to dry eye disease.

Research

JoVE Journal - Medicine
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Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres

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Cited by 25 •

2015

We present a method for inducing elevated intraocular pressure (IOP), by injecting magnetic microspheres into the rat eye, to model glaucoma. This leads to strong pressure rises, and extensive neuronal death. This protocol is easy to perform, does not require repeat injections, and produces stable long-lasting IOP rises.

Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography

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Cited by 6 •

2024

Here, a normal-phase, high-performance liquid chromatography method is described to detect and quantify critical retinoids involved in the facilitation of visual function in both ocular and systemic tissue, in the context of the systemic vitamin A supply to generate the essential photosensitive rhodopsin chromophore 11-cis-retinal.

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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Cited by 15 •

2013

We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

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Cited by 9 •

2020

Here, we describe the use of spectral-domain optical coherence tomography (SD-OCT) to visualize retinal and ocular structures in vivo in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia.

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