Pbs Washing Step
Pbs Washing Step - Related Videos
00:52
Washing, Drying, and Ignition of Precipitates
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2024
After filtration, the precipitate is washed to remove coprecipitated impurities and any remaining mother liquor. Colloidal precipitates, such as silver chloride, are washed with an electrolyte (such as dilute nitric acid) to prevent the peptization of the precipitate. In the case of slightly soluble precipitates, the wash solution contains a common ion to reduce solubility. Lead sulfate, which is slightly soluble in water, is washed with dilute sulfuric acid. Similarly, wash solutions may be...
06:46
Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature
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Cited by 9 •
2019
A compact pulsed laser diode-based desktop photoacoustic tomography (PLD-PAT) system is demonstrated for high-speed dynamic in vivo imaging of small animal cortical vasculature.
12:31
A Step by Step Protocol for Subretinal Surgery in Rabbits
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Cited by 30 •
2016
Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.
06:39
A Step-by-Step Guide to Mosquito Electroantennography
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Cited by 6 •
2021
The present article details a step-by-step protocol for successful and low noise electroantennograms in several genera of mosquitoes, including both females and males.
12:25
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
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Cited by 23 •
2012
Nanodiscs are small discoid particles that incorporate membrane proteins into a small patch of phospholipid bilayer. We provide a visual protocol that shows the step-by-step incorporation of the MalFGK2 transporter into a disc.
FAQs
PBS helps maintain a near-physiological ionic environment and a stable pH while fluid around the sample is exchanged. Those conditions reduce abrupt osmotic or acid-base changes that could compromise sample integrity during rinsing. This chemical stability is especially important when cells or biological material must remain suitable for later staining, microscopy, culture-related handling, or molecular analysis.
Removing unbound reagents, residual media, proteins, and debris lowers the amount of material that can remain during a subsequent assay stage. This can reduce nonspecific background and limit carryover, meaning unwanted material is transferred into the next step. Cleaner sample conditions help distinguish the intended signal from interference, supporting more reliable staining, imaging, and molecular assay results.
Adequate PBS coverage and gentle handling are central to a consistent wash. Coverage allows the buffer to contact the relevant sample surfaces or cells, while gentle manipulation helps preserve sample integrity. If either factor is poorly controlled, unwanted substances may remain or the sample may be disturbed, reducing signal quality and making results less reproducible across experiments.
The sample is exposed to PBS so the relevant surface or cell suspension receives adequate coverage, then the wash liquid is removed to carry away displaced media, proteins, residual reagents, or debris. Handling should remain gentle throughout. The goal is complete contact and effective removal without compromising the sample before the next protocol stage.
It can be used between stages of procedures in which residual material could interfere with the next reaction or observation. In the supported biology applications, washing helps prepare cultured cells or biological samples for immunostaining, microscopy, and molecular assays. Its value is greatest when reducing background or carryover improves interpretation of the resulting signal.
The step supports reliability by combining chemical stability with removal of unwanted material. Maintaining suitable ionic conditions and pH helps protect the sample, while clearing residual media, proteins, reagents, and debris reduces background. Together, these effects can improve sample integrity, signal quality, and reproducibility, allowing results from imaging or molecular assays to be interpreted more confidently.