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JoVE Journal
Biology

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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
 

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Article DOI: 10.3791/3910
August 31st, 2012

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Summary August 31st, 2012

Nanodiscs are small discoid particles that incorporate membrane proteins into a small patch of phospholipid bilayer. We provide a visual protocol that shows the step-by-step incorporation of the MalFGK2 transporter into a disc.

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