Peristaltic Pump Perfusion
Peristaltic Pump Perfusion - Related Videos
04:21
Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump
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2025
Source: Léa Rubira1, Hélène Sturer1, Louise Combette1, Sandra Santini1, Stéphane Renaud1, Cyril Fersing1,21Nuclear medicine department, Institut régional du Cancer de Montpellier (ICM), Univ Montpellier, 2IBMM, Univ Montpellier, CNRS, ENSCMThis video demonstrates the safe, controlled infusion of a radiopharmaceutical using a peristaltic pump with a dual-line cassette system. The setup includes connecting saline and infusion lines, positioning the shielded vial, and initiating simultaneous...
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
06:05
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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Cited by 3 •
2017
Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.
11:23
An Open-Source Normothermic Perfusion System Designed for Research Scientists
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Cited by 2 •
2025
Here, we present a protocol for an open-source, modifiable normothermic ex vivo perfusion system designed to overcome the high cost and limited flexibility of existing platforms, enabling more accessible, cost-efficient, and iterative use in pre-clinical biomedical research.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
FAQs
As the rotating rollers compress flexible tubing, each point of occlusion moves along the tube in sequence. This creates a traveling pressure pattern that pushes culture medium forward while the tubing behind the occlusion returns to its open state. Because the rollers do not contact the medium directly, the fluid pathway remains separated from the mechanical drive system.
Flow rate can be adjusted primarily through roller speed and tubing dimensions. Increasing or decreasing roller speed changes how rapidly occlusions move through the tubing, while tubing dimensions influence the amount of medium displaced during each cycle. Controlling these variables helps regulate nutrient and oxygen delivery through a tissue construct, organoid, or bioreactor.
Continuous circulation supports ongoing delivery of nutrients and oxygen while carrying waste away from the cultured system. This improves mass transfer compared with relying only on local diffusion and helps maintain conditions suitable for extended culture. The resulting flow also provides a more dynamic environment for studying biological systems than a completely static medium arrangement.
A typical setup connects a flexible tubing pathway between the culture system and medium reservoirs, positions the tubing within the roller mechanism, and establishes circulation through the biological material. The operator then selects roller speed and suitable tubing dimensions to obtain the intended flow. Maintaining a closed pathway supports sterile operation while medium enters and exits continuously.
Researchers may choose Peristaltic Pump Perfusion when cultures require sustained nutrient and oxygen transport, waste removal, or conditions that better represent a dynamic physiological environment. It is relevant to long-term culture of tissue constructs and organoids, where continuous medium movement can support mass transfer and help maintain experimental conditions over extended periods.
In biology, the method can support studies of tissue engineering and disease research by exposing cultured systems to controlled, continuously refreshed medium. It helps researchers examine how tissue constructs, organoids, or bioreactor cultures respond when nutrient delivery, oxygen transport, and waste removal occur over time rather than through a single static medium exchange.