An Open-Source Normothermic Perfusion System Designed for Research Scientists
1.9K views
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Cited by 2
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11:23 min
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July 18th, 2025
DOI :10.3791/68722-v
July 18th, 2025
•1.9K views
Lindsay R. Friedman1 , Hannah E. Stepp1 , Jacob Lambdin1 , Allen Luna1 , Alexander Rossi1 , Stephanie C. Lux1 , Michael M. Wach1 , Kenneth Luberice1 , Samantha M. Ruff1 , James McDonald1 , Reed I. Ayabe1 , Shahyan U. Rehman1 , Carrie Ryan1 , Stephanie Gregory1 , Alyssa V. Eade1 , Carolina Larrain1 , Jack H. Victory1 , Randy Pursley2 , Taylor Noel2 , Max Freidlin2 , Shreya Gupta1 , Tracey Pu1 , Ashley Rainey1 , Emily Verbus1 , Martha Teke1 , Areeba Saif1 , Tahsin M. Khan1 , Imani A. Alexander1 , Surajit Sinha1 , Sarfraz R. Akmal1 , Ritish R. Maram3 , Leila Sarvestani1 , Stephen M. Hewitt4 , James Doroshow5 , Kirsten Remmert1 , Emily Smith1 , Priyanka Desai1 , Ning Miao6 , Kevin Driscoll6 , Kamille West7 , Theo Heller8 , Elliot Levy3 , Jeremy Davis1 , Andrew Blakely1 , David E. Kleiner4 , John Kakareka2 , Thomas Pohida2 , Jonathan M. Hernandez1,9
1Surgical Oncology Program, National Cancer Institute, National Institutes of Health, 2Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 3Radiology and Imaging Sciences, Department of Interventional Radiology, National Institutes of Health, 4Histopathology and Autopsy Pathology, Laboratory of Pathology, National Institutes of Health, 5Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, 6Department of Perioperative Medicine, National Institutes of Health, 7Blood Services Section, Department of Transfusion Medicine, National Institutes of Health, 8Translational Hepatology Section, Liver Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9Center for Immuno-Oncology, National Cancer Institute, National Institutes of Health
Here, we present a protocol for an open-source, modifiable normothermic ex vivo perfusion system designed to overcome the high cost and limited flexibility of existing platforms, enabling more accessible, cost-efficient, and iterative use in pre-clinical biomedical research.
Chapters in this video
0:00
Introduction
0:50
Machine Set-Up Normothermic Ex Vivo Perfusion System
9:29
Results
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