Plant Cell Culture
Plant Cell Culture - Related Videos
02:57
Plant Tissue Culture
0 Views •
2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
03:03
Preparation of Agrobacterium Culture for Gene Delivery into Plant Cells
0 Views •
2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
12:30
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
0 Views •
Cited by 26 •
2012
A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.
07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
0 Views •
Cited by 26 •
2014
Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.
08:39
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
0 Views •
Cited by 6 •
2017
Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.
FAQs
Cellular totipotency allows plant cells to retain the capacity to regenerate into organized structures, including shoots, roots, or whole plants. In culture, researchers use this capacity alongside controlled hormone concentrations and environmental conditions to direct development. This principle explains why a small explant can support multiplication, regeneration, and the recovery of complete plants.
Plant growth regulators help determine whether cultured cells proliferate as callus, grow as suspension cultures, or proceed toward shoot and root formation. Their concentrations can be adjusted in the nutrient medium to alter developmental outcomes. This flexibility makes hormone control central to coordinating cell multiplication with the regeneration stage required for a particular biological or biotechnological objective.
Callus cultures consist of proliferating, relatively unorganized plant cells or tissues, whereas suspension cultures maintain proliferating cells in a liquid-based culture context. Both forms support cell multiplication, but they provide different experimental systems for studying growth and producing biological materials. Subsequent adjustment of hormones and conditions can promote regeneration of shoots, roots, or whole plants.
Sterile conditions and environmental control allow researchers to maintain plant cells, tissues, or organs under defined growth conditions rather than uncontrolled surroundings. The nutrient medium supplies minerals, sugars, and vitamins, while environmental adjustments work with plant growth regulators to influence proliferation and regeneration. Such control makes experimental outcomes easier to associate with selected culture conditions.
A typical workflow begins with selecting a small plant explant and placing it on a nutrient medium containing minerals, sugars, vitamins, and plant growth regulators. Researchers then maintain the material under sterile, controlled conditions and adjust hormones or environmental factors as needed. The culture may proliferate as callus or suspension cells before being directed toward shoots, roots, or whole plants.
Plant Cell Culture supports micropropagation by enabling researchers to multiply plant material and regenerate plants under controlled conditions. It also helps conserve rare plant genetic resources, extending its value beyond routine propagation. These uses are especially relevant when the goal is to maintain valuable plant material, support crop improvement, or preserve genetic resources that may require specialized handling.
The technique supports research on plant development, stress responses, and plant-microbe interactions while also enabling production of useful biological materials and valuable metabolites. Its controlled setting helps connect culture conditions with cellular growth and developmental outcomes. In biotechnology and crop improvement, the same platform can contribute to regeneration, material production, and investigation of plant traits.