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Biology

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Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
 

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Article DOI: 10.3791/55995 08:39 min December 24th, 2017
December 24th, 2017

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Summary

Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.

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Live-cell Imaging Fungal Cells Modes Of Entry Subcellular Localization Antifungal Plant Defensins Confocal Microscopy Internalization Cell Organelles Cytoplasm Conidial Suspension Fusarium Graminearum Carboxymethyl Cellulose Medium Rotary Shaker Red Color Filtration Material Microcentrifuge Tube
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