Respirometric Assessment
Respirometric Assessment - Related Videos
11:10
Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
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Cited by 32 •
2011
Saponin-permeabilized fiber preparation in conjunction with respirometric oxidative phosphorylation analysis provides integrative assessment of mitochondrial function. Mitochondrial respiration in physiological and pathological states can reflect various regulatory influences including mitochondrial interactions, morphology and biochemistry.
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Cited by 24 •
2015
Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
08:11
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
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Cited by 2 •
2022
Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.
08:03
Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
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Cited by 1 •
2025
This study introduces a methodology for studying xenobiotic effects on mitochondrial function via respirometric and enzymatic assays. It examines electron transport chain modulation in various species, offering insights for targeted biopesticide development against insect vector-borne diseases.
08:36
Neurotoxicity Assessment in Adult Danio rerio using a Battery of Behavioral Tests in a Single Tank
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Cited by 3 •
2023
Here, we present a comprehensive behavioral test battery, including the novel tank, Shoaling, and social preference tests, to effectively determine the potential neurotoxic effects of chemicals (e.g., methamphetamine and glyphosate) on adult zebrafish using a single tank. This method is relevant to neurotoxicity and environmental research.
FAQs
The measured gas signal serves as an indicator of biological energy metabolism. Oxygen consumption, carbon dioxide production, or other changes in gas composition can be tracked separately or together, depending on the respirometer setup. Comparing these signals over time helps quantify respiration and identify differences in metabolic activity between samples or between experimental conditions.
A sealed respirometer monitors gas changes within an enclosed chamber, whereas a flow-through system measures gas composition as material moves through the chamber. This distinction affects how gas exchange is observed and how experimental conditions are controlled. The choice of configuration therefore depends on whether the study requires enclosed monitoring or measurement during continuous gas movement.
Temperature, substrate availability, environmental stress, toxins, and changing physiological demands can all influence the measured rate. Because these factors affect biological activity, researchers control or vary them to determine how metabolism responds. Consistent conditions support comparisons among samples, while deliberate changes help reveal sensitivity to nutrients, stressors, or physiological state.
A respiration rate converts gas exchange into a quantitative measure of metabolic activity. Researchers can compare rates across samples or conditions to evaluate differences in cellular or organismal performance. Such comparisons may show altered activity associated with microbial growth, mitochondrial function, environmental stress, nutrient availability, or other changes in biological demand.
A typical assessment places the biological sample in a sealed or flow-through chamber, connects the chamber to sensors that monitor gas composition, and controls relevant conditions such as temperature and substrate availability. The recorded oxygen, carbon dioxide, or related gas changes are then used to determine respiration rate and compare the resulting metabolic responses.
The method is useful when researchers need quantitative evidence of biological energy metabolism. Applications include examining microbial growth, mitochondrial function, soil activity, aquatic organisms, and ecosystem processes. It can also evaluate responses to environmental stress, nutrients, toxins, or changing physiological demands, allowing metabolic activity to be compared across biological systems.
Measurements can reveal whether metabolic activity changes under different experimental conditions and whether samples differ in performance. In biological studies, the results may indicate responses linked to growth, stress, nutrient availability, toxins, or physiological demand. At larger scales, the same approach supports analysis of soil, aquatic, and ecosystem-level respiration processes.