Sample Mounting
Sample Mounting - Related Videos
04:28
Light Sheet Microscopy Sample Preparation: Mounting Live Zebrafish Embryos for Long-Term Imaging
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2023
This article describes sample preparation for light-sheet fluorescence microscopy. It also demonstrates a method to mount live zebrafish embryos for long term imaging, which allows recording of long time-lapse movies of live embryos in near-physiological conditions.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
04:32
Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
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2023
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
09:42
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
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Cited by 4 •
2017
Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.
06:36
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Cited by 50 •
2014
The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.
FAQs
The mounting medium helps maintain the specimen’s structure while supporting controlled optical conditions. By surrounding the sample beneath the coverslip, it can reduce optical distortion and limit direct air exposure. This contributes to clearer examination of cells, tissues, microorganisms, and stained sections, especially when preserving structural detail is important.
A coverslip helps protect the prepared specimen and contributes to its stable positioning for microscopy. It also helps reduce movement and air exposure, two conditions that can interfere with observation. Careful placement therefore supports more consistent images and helps preserve the sample for later documentation or comparison.
Thin samples are better suited to controlled optical examination because they allow the microscope to observe specimen features without unnecessary material obstructing the view. Alignment on the slide further supports consistent positioning beneath the coverslip. Together, these factors help reduce optical distortion and improve image quality across biological preparations.
The preparation begins by placing a thin biological sample in a mounting medium on a microscope slide. The specimen is then aligned, and a coverslip is placed over it to protect and stabilize the preparation. This sequence creates a controlled setup for examining cells, tissues, microorganisms, or stained sections with light microscopy.
Sample Mounting is useful when researchers need to observe biological structures while preserving specimen organization and limiting movement. It supports light-microscopy examination of cells, tissues, microorganisms, and stained sections. The resulting preparations can also help researchers document samples and compare observations more reliably across experiments.
Consistent mounting conditions help place specimens in a comparable optical setup, which supports more reliable observations across experiments. Preserving structure and reducing movement can make images easier to document and interpret. This is particularly valuable when researchers compare biological samples or record microscopy findings for later reference.