Stereotaxic Atlas
Stereotaxic Atlas - Related Videos
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
00:08
Survivable Stereotaxic Surgery in Rodents
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Cited by 50 •
2008
The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.
02:24
Stereotaxic Cisterna Magna Approach for Therapeutic Delivery in a Mouse Model
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2025
Source: Joshi, K., et al. Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice. J. Vis. Exp. (2022)This video demonstrates the cisterna magna stereotaxic approach for targeted delivery to the brainstem and upper spinal cord. By accessing the cerebrospinal fluid-filled cisterna magna, this approach enables precise injections into areas that are difficult to reach using standard stereotaxic methods.
03:38
Developing a Cerebral Blood Flow Atlas for Blood Flow Mapping
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2025
Source: Tan, Z., et al., Construction and Application of Cerebral Functional Region-Based Cerebral Blood Flow Atlas Using Magnetic Resonance Imaging-Arterial Spin Labeling. J. Vis. Exp. (2024).This video demonstrates the method to construct a cerebral blood flow atlas by integrating magnetic resonance imaging or MRI-arterial spin labeling data with segmented brain regions, visualizing blood flow patterns, and quantifying flow levels across functional areas.
09:09
Dissecting the Non-human Primate Brain in Stereotaxic Space
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Cited by 14 •
2009
The non-human primate is an important translational species for our understanding of the normal processing of the brain. The anatomical organization of the primate brain can provide important insights into normal and pathological conditions in humans.
FAQs
Coordinates convert a three-dimensional location into a position that can be referenced across a brain map. The atlas relates that position to stable landmarks, including the skull, ventricles, or other consistent anatomical features. Matching an experimental target to these references helps researchers localize placements rather than relying on visual estimates alone.
Serial tissue sections and imaging data provide complementary ways to construct or consult the map. Their alignment organizes observations from separate slices or image planes within one three-dimensional coordinate framework. This allows a structure seen in one section or dataset to be related to neighboring anatomy and to a coordinate location used for experimental planning.
Standardization makes anatomical observations comparable across animals and laboratories. When findings are expressed relative to the same coordinate framework and anatomical landmarks, researchers can evaluate whether placements or sampled regions correspond to similar structures. This supports reproducibility and makes it easier to relate neuroanatomical results to neural circuits, behavior, disease mechanisms, or connectivity.
Researchers first identify the anatomical target and locate its corresponding position in the atlas. They then use the atlas coordinates and relevant landmarks to guide the planned intervention or sampling, such as an injection, electrode placement, tissue collection, or lesion. Afterward, the mapped location provides a basis for interpreting the experimental result.
An atlas-based location helps researchers determine which anatomical structure was affected or sampled and relate that site to the study's biological question. For example, a placement can be interpreted in relation to a neural circuit, while a lesion or tissue sample can be compared with mapped anatomy. The coordinate record also supports cross-study comparison.
Within biology, stereotaxic atlases support experiments on neural circuits, behavior, disease mechanisms, and brain connectivity. They are especially useful when a study requires a spatially precise intervention or sample and when results must be compared across animals or laboratories. The atlas therefore links physical location with experimental design and broader neuroanatomical interpretation.