Surfactant Removal
Surfactant Removal - Related Videos
09:34
Extraction and Characterization of Surfactants from Atmospheric Aerosols
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Cited by 26 •
2017
Methods are presented for the targeted extraction of surfactants present in atmospheric aerosols and the determination of their absolute concentrations and surface tension curves in water, including their Critical Micelle Concentration (CMC).
07:20
Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)
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Cited by 21 •
2016
Repeated pulmonary lavages in anesthetized pigs induce lung injury resembling major aspects of human acute respiratory distress syndrome (ARDS). For this purpose the lungs are repeatedly lavaged with 0.9% saline at 37 °C. The goal of the protocol is a reproducible mitigation of gas exchange and hemodynamics for research in ARDS.
10:28
Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
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Cited by 15 •
2014
This paper demonstrates the experimental procedure to measure terminal settling velocities of spherical particles in surfactant-based shear thinning viscoelastic fluids. Fluids over a wide range of rheological properties are prepared and settling velocities are measured for a range of particle sizes in unbounded fluids and fluids between parallel walls.
06:35
Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water
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2025
This study presents the methodology for the generation of six different types of plasma discharges within a Hyperbolic Vortex Plasma Reactor for the degradation of micropollutants in water, including pharmaceuticals and per- and polyfluoroalkyl substances (PFAS).
07:11
Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
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Cited by 2 •
2016
Biofilms on surfaces can be effectively and rapidly removed by using a periodic jet of carbon dioxide aerosols without a nitrogen purge.
FAQs
The surfactant’s chemical properties guide method selection because different approaches exploit different separation behaviors. Size differences favor dialysis or ultrafiltration, while solubility can support phase separation. Charge or affinity for a binding material can make adsorption useful. Concentration also matters, since the amount present influences which approach is suitable for the biological material and the intended downstream use.
Residual surfactant can alter membranes, proteins, and cellular behavior, so an otherwise suitable sample may perform differently in a downstream experiment. In protein work, remaining molecules may compromise preservation of protein structure. In cell-based studies, they can change cellular responses. Removing them helps reduce experimental interference and supports more reliable interpretation of biological assays.
These methods separate surfactants through different physical or chemical bases rather than serving as interchangeable steps. Dialysis and ultrafiltration use size-related separation, adsorption depends on affinity for a binding material, and phase separation uses differences in solubility. Dilution lowers the surfactant level without relying on a single binding or partitioning mechanism. The appropriate comparison depends on sample sensitivity and surfactant properties.
Before selecting a Surfactant Removal approach, consider the surfactant’s concentration and properties, including size, solubility, charge, and affinity for a binding material. Then weigh how sensitive the sample is to treatment, particularly if it contains proteins, membranes, or cells. This matching process helps balance effective removal with preservation of protein structure and compatibility with later biological assays.
A practical workflow begins by characterizing the surfactant and identifying what must be preserved in the sample. Researchers can then choose dilution, dialysis, ultrafiltration, adsorption, or phase separation according to the relevant separation property. The selected treatment should be considered in relation to the intended downstream assay, because a method that removes surfactant may still be unsuitable if it disrupts the biological material.
It is particularly relevant when surfactants could affect protein structure, membrane behavior, or cellular responses before analysis. Removal can improve the suitability of protein preparations for downstream assays and reduce unwanted effects in cell-based experiments. The same principle applies across biological samples, formulations, and experimental systems, but the method must be adapted to surfactant concentration and sample sensitivity.