Telomerase Rna Component
Telomerase Rna Component - Related Videos
02:41
Telomeres and Telomerase
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2020
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
02:41
Telomeres and Telomerase
0 Views •
2023
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
09:25
In vitro Reconstitution of the Active T. castaneum Telomerase
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Cited by 5 •
2011
Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more than a decade. Here, we present methods for the isolation of the recombinant Tribolium castaneum TERT (TcTERT) and the reconstitution of the active T. castaneum telomerase ribonucleoprotein (RNP) complex in vitro.
12:08
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Cited by 29 •
2013
An accurate, short, sophisticated and cheap method is described that assesses telomere length in multiple tissues and species using qRT-PCR. In addition, we will describe a simple assay to assess telomerase activity as a complementary backbone test for telomere length.
08:26
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Cited by 5 •
2018
Human telomerase reverse transcriptase (TERT) synthesizes not only telomeric DNA but also double-stranded RNA through RNA-dependent RNA polymerase activity. Here, we describe a newly established assay to detect RNA-dependent RNA polymerase activity of endogenous TERT.
FAQs
Its template region provides sequence information that telomerase reverse transcriptase uses when adding repeated DNA to chromosome ends. This guided synthesis helps offset the sequence loss that accompanies chromosome replication. The RNA therefore contributes both informational specificity and continuity to telomere maintenance, supporting preservation of chromosome-end integrity in cells that divide repeatedly.
The RNA component supplies the template, while telomerase reverse transcriptase carries out the DNA-synthesis step. Neither role alone provides the complete telomere-maintenance function described for telomerase. Their partnership links sequence guidance with enzymatic addition of repeats, enabling the system to compensate for replication-associated loss at chromosome ends and help preserve genome stability.
Structure and regulation determine how effectively the RNA component supports telomerase function. Alterations in either feature could change the component’s ability to participate in template-guided repeat addition, thereby influencing telomere maintenance. Examining these properties helps researchers connect molecular changes with differences in chromosome-end preservation, sustained proliferation, or disease-associated telomerase behavior.
Cells requiring sustained proliferation, including many stem and germline cells, depend particularly on effective telomere maintenance. Repeated division increases the importance of compensating for sequence loss at chromosome ends. Studying the RNA component in these cell types helps clarify how telomerase supports continued proliferation while contributing to the preservation of genome stability.
Research commonly focuses on the component’s molecular structure, regulation, and activity. These dimensions provide complementary information: structure addresses how the RNA supports telomerase function, regulation addresses when that function is controlled, and activity addresses its contribution to telomere maintenance. Together, they help investigators interpret how chromosome ends are preserved in relevant biological settings.
Its role in telomere maintenance connects the component to several biological and medical research areas. Studying it can support investigation of cellular aging and inherited telomere disorders, where telomere preservation is important, while cancer research examines abnormal telomerase activation that may promote prolonged cell survival. These contexts make its regulation and activity especially significant.