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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

DOI:
10.3791/50246-v

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12:08 min

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May 22, 2013

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, ,
1Department of Medicine,Albert Einstein College of Medicine , 2Diabetes Research and Training Center,Albert Einstein College of Medicine , 3Department of Genetics,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 01:49Telomere Length Measurement using Quantitative Real-Time PCR
  • 05:50Quantitative Telomerase Detection Activity Measurement
  • 09:47Results: Quantitative Real-Time PCR is Used to Determine Telomere length and Telomerase Activity in Human Leukocytes
  • 11:41Conclusion

Summary

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An accurate, short, sophisticated and cheap method is described that assesses telomere length in multiple tissues and species using qRT-PCR. In addition, we will describe a simple assay to assess telomerase activity as a complementary backbone test for telomere length.

Tags

Keywords: Telomere LengthTelomerase ActivityCell SenescenceChromosome AttritionCell DivisionCell AgingQRT-PCRTelomere SequenceTelomere AssayTelomerase Assay

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