Three-dimensional Cell Culture
Three-dimensional Cell Culture - Related Videos
02:02
A Three-Dimensional Co-culture Model of Infiltrating T Cells in Tumor Spheroids
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2025
This video demonstrates a method to establish a three-dimensional tumor spheroid and T cell co-culture. An agarose cast with microwells containing tumor spheroids is prepared, followed by the addition of T cells. After incubation, this setup allows studying the interaction of T cells with tumor spheroids in a controlled three-dimensional environment.
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Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
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Cited by 16 •
2012
A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.
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Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells
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Cited by 5 •
2016
Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.
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Three-Dimensional Culture Systems: Spheroids and Organoids
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2026
Three-dimensional (3D) culture systems, including spheroids and organoids, support New Approach Methodology (NAM)-aligned research by providing more physiologically relevant models than conventional two-dimensional cell cultures. Because they more closely resemble the structure and cellular interactions of tissues and organs, these systems can provide physiologically relevant data before advancing to animal studies. Spheroids as Disease and Drug Screening Models Spheroids are generated when one...
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
FAQs
As cells become spatially distributed through a three-dimensional construct, oxygen and nutrient availability can vary with location. These gradients influence signaling, viability, growth, and drug responses, so cells in different regions may behave differently under the same experimental condition. This makes spatial organization an important factor when interpreting culture behavior and treatment effects.
Cell-cell interactions and cell-matrix interactions provide distinct signals that shape growth, differentiation, and spatial organization. In a three-dimensional setting, these relationships occur throughout the culture rather than only across a flat layer. Their combined effects help explain why cells can display biological behaviors and responses that differ from those observed in two-dimensional cultures.
Scaffolds and extracellular matrix materials provide a surrounding structure, while spheroids and organoids organize cells into multicellular assemblies. These formats are not interchangeable in how they create spatial relationships. Choosing among them therefore affects how researchers model tissue organization, study differentiation, or examine responses to experimental treatments.
At a broad level, a setup begins by selecting a culture format, such as a scaffold or extracellular matrix material, a spheroid, or an organoid. Cells are then established within that three-dimensional arrangement, where their interactions and spatial position can be examined. The chosen format should match whether the study emphasizes tissue organization, differentiation, disease mechanisms, or treatment response.
This approach supports investigations of development, disease mechanisms, tissue regeneration, and therapeutic testing. Its value comes from allowing researchers to examine how spatial organization, cell interactions, and local oxygen or nutrient conditions influence biological behavior. These features make it useful when a study requires tissue-like organization or treatment responses that may not be represented adequately in a flat culture.
Comparing the two formats can reveal how spatial organization and cell-cell or cell-matrix interactions affect experimental outcomes. Three-dimensional cultures may produce more physiologically relevant results because they generate tissue-like relationships and gradients that are absent or different in flat layers. Such comparisons can clarify whether growth, differentiation, signaling, viability, or drug responses depend on culture geometry.