Tip Ejection

Tip ejection is the controlled removal of a disposable pipette tip from a pipette, an essential step for efficient and contamination-conscious liquid handling in biology. In a manual pipette, pressing the tip-ejector button drives an internal sleeve or rod downward, releasing the tip from the nozzle without requiring direct contact. Proper tip ejection supports rapid transfer between samples, reduces exposure to potentially hazardous liquids, and helps prevent cross-contamination of reagents and specimens. It is used routinely in molecular biology, microbiology, cell culture, clinical research, and automated liquid-handling workflows, where consistent disposal contributes to reliable experimental results.

Tip Ejection - Related Videos

Research

JoVE Journal - Medicine
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A Murine Model of Hyperlipidemia-Induced Heart Failure with Preserved Ejection Fraction

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Cited by 2 •

2024

This protocol presents a detailed approach to replicating a murine model of hyperlipidemia-induced heart failure with preserved ejection fraction (HFpEF). The design combines the administration of adeno-associated virus 9-cardiac troponin T-low-density lipoprotein receptor (AAV9-cTnT-LDLR) and poloxamer-407 (P-407).

Research

JoVE Journal - Medicine

A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs

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Cited by 5 •

2022

The present protocol describes a step-by-step procedure to establish a minipig model of heart failure with preserved ejection fraction using descending aortic constriction. The methods for evaluating cardiac morphology, histology, and function of this disease model are also presented.

Lumped-Parameter and Finite Element Modeling of Heart Failure with Preserved Ejection Fraction

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Cited by 9 •

2021

This work introduces two computational models of heart failure with preserved ejection fraction based on a lumped-parameter approach and finite element analysis. These models are used to evaluate the changes in the hemodynamics of the left ventricle and related vasculature induced by pressure overload and diminished ventricular compliance.

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

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Cited by 6 •

2018

Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

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Cited by 10 •

2019

We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

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