Tubulin Expression
Tubulin Expression - Related Videos
02:40
Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects
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2025
Source: Piazza, V., et al., Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (2023)This video demonstrates the procedure for imaging microtubule abnormalities in a rat brain tissue slice using a two-photon excitation microscope. It employs second harmonic generation (SHG) signals to detect tubulin defects and reduced myelin production.
06:30
Optimizing Tubulin Yield from Porcine Brain Tissue
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2024
This protocol describes a technique for the high-yield isolation of tubulin from the porcine brain optimized for small-scale instrumentation. The isolation procedures are complemented by procedures for determining tubulin polymerization activity in vitro using co-sedimentation assays and transmission electron microscopy.
08:07
Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
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2023
In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
07:54
Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
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Cited by 5 •
2020
This protocol describes tubulin purification from small/medium-scale sources such as cultured cells or single mouse brains, using polymerization and depolymerization cycles. The purified tubulin is enriched in specific isotypes or has specific posttranslational modifications and can be used in in vitro reconstitution assays to study microtubule dynamics and interactions.
FAQs
Tubulin expression connects gene activity with cytoskeletal assembly through sequential steps. Tubulin genes are transcribed into messenger RNAs, which are translated into tubulin proteins. These proteins then form α/β-tubulin heterodimers, the units that assemble into microtubule polymers. Examining these stages helps distinguish changes in gene output from later changes in microtubule organization.
Cells coordinate tubulin production with microtubule assembly because cytoskeletal demands change during development and under different physiological conditions. Altering expression can influence the supply of tubulin available for polymer formation, while assembly determines how that material contributes to cellular organization and movement. This coordination is therefore important when interpreting changes in cell structure or behavior.
α/β-tubulin heterodimers provide the immediate building units for microtubule polymers. Their formation links the production of individual tubulin proteins to the higher-order cytoskeletal structures that organize the cell. Measuring expression without considering this assembly step may not fully explain the resulting microtubule state, because cellular organization also depends on how tubulin enters dynamic polymers.
Researchers can examine tubulin expression using quantitative PCR, immunoblotting, or immunostaining. These approaches support analysis of tubulin-related changes in biological samples and can be applied to questions about cytoskeletal organization, cell division, neuronal development, or drug response. Using more than one method can help relate expression measurements to cellular outcomes studied in the same system.
Tubulin expression analysis is useful when researchers need to assess how cells organize their cytoskeleton during division or neuronal development. Because tubulin contributes to microtubule structures involved in cell organization and movement, expression measurements can provide evidence of cytoskeletal regulation in these settings. The results support comparisons between developmental, physiological, or otherwise experimentally distinct cellular conditions.
Measuring tubulin expression helps researchers examine cellular responses to microtubule-targeting drugs and investigate diseases associated with altered cytoskeletal regulation. Changes detected through quantitative PCR, immunoblotting, or immunostaining can be evaluated alongside effects on cell division, neuronal development, or cytoskeletal organization. This connects molecular measurements with broader biological consequences of disturbed microtubule regulation.