Basal Epithelial Cells
Basal Epithelial Cells - Related Videos
11:52
Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
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Cited by 17 •
2012
Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Cited by 135 •
2013
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
FAQs
Basal epithelial cells attach to the extracellular matrix at the tissue base, linking their position to maintenance of epithelial structure. This attachment provides the physical context in which they divide and produce replacement cells. In research, examining whether normal and transformed cells retain comparable attachment helps evaluate changes in tissue organization during tumor development.
Symmetric division can expand the basal cell population, whereas asymmetric division can balance self-renewal with production of cells that move toward differentiated epithelial layers. This distinction helps researchers examine whether a cell population primarily maintains its source compartment or supplies new tissue. Comparing these division patterns can clarify progenitor or stem-like behavior in normal and transformed cells.
Researchers compare proliferation, differentiation, tissue organization, and signaling pathways. A transformed population may show altered behavior in one or more of these dimensions, making the comparison useful for identifying changes associated with early tumor development. The value of the model lies in connecting cellular changes with disrupted epithelial renewal and organization rather than examining proliferation alone.
A study can establish a normal reference state, examine basal-cell renewal and differentiation, and compare those observations with transformed cells. Researchers then assess differences in proliferation, tissue organization, and signaling pathways. This progression connects baseline epithelial biology to early cancer-associated changes and can help identify which altered behaviors merit further investigation.
These experiments can show how changes in a basal epithelial population affect renewal, differentiation, and organization, while also revealing altered signaling pathways. Such results support questions about where tumors originate and which cellular features distinguish normal from transformed states. They may also help researchers investigate potential biomarkers or develop ideas for therapeutic strategies based on differences between the two conditions.
Because they can divide symmetrically or asymmetrically and generate cells that migrate and differentiate, basal epithelial cells provide a system for examining how renewal is balanced with specialization. In cancer research, this framework allows investigators to ask whether transformed cells preserve, alter, or disrupt behaviors associated with epithelial stem or progenitor populations.