Immune Cell Imaging
Immune Cell Imaging - Related Videos
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
04:28
Analysis of Immune Synapses between Human T Cells and SEB-Loaded Raji Cells using Imaging Flow Cytometry
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2025
This video analyzes the immune synapse formation between T cells in unpurified pan-leukocyte preparations and Staphylococcus aureus enterotoxin B, SEB-loaded Raji cells. CD3 and F-actin enrichment is observed in the immune synapse using imaging flow cytometry.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
04:02
Fluorescence Imaging of Neuro-Immune Dynamics Using a Skull Implant
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2025
Source: Bisht, K., et al. Precise brain mapping to perform repetitive in vivo imaging of neuro-immune dynamics in mice. J. Vis. Exp. (2020)This video demonstrates fluorescence imaging of neuro-immune dynamics in a transgenic mouse with a skull implant. Vascular and neuronal stability is imaged alongside dynamic microglial changes, reflecting immune responses to neuronal activity.
FAQs
Fluorescent labels, reporter proteins, and antibody-based probes provide detectable signals that microscopy can associate with particular immune cells or cellular states. When these signals are followed across tissue regions or over time, investigators can examine movement, activation, infiltration, and cell-cell contact rather than relying only on a final measurement. This makes the immune response spatially interpretable.
The most informative readout depends on which interaction is being examined. Cell movement indicates how immune cells navigate tissue, infiltration shows whether they enter tumor regions, and contact with tumor or stromal cells identifies physical encounters. Activation adds a functional dimension, helping researchers connect location and behavior with how immune cells respond inside the tumor microenvironment.
Imaging links immune-cell behavior to the local tumor microenvironment, including neighboring malignant and stromal cells. That spatial context helps distinguish whether immune cells can reach a tumor, recognize malignant cells, and respond after arriving. Consequently, the method can expose differences between immune-cell presence alone and evidence of interaction or activation within cancer tissue.
A basic workflow begins by selecting a fluorescent label, reporter protein, or antibody-based probe that can make the immune-cell population or feature of interest visible. Researchers then combine the signal with microscopy and examine tissue for distribution, movement, activation, infiltration, or contacts. The resulting images are interpreted in relation to tumor and stromal locations.
In immunotherapy studies, imaging can compare immune-cell distribution and function before and after treatment or between treatment conditions, when those comparisons are built into the experiment. Changes in where cells accumulate, whether they infiltrate tumors, and how they interact with malignant or stromal cells can indicate altered immune engagement. These observations help evaluate treatment effects.
Cancer researchers use these measurements to investigate tumor targeting and the relationship between immune responses and treatment performance. Evidence of immune-cell access, recognition, contact, or activation can help explain why an intervention changes the tumor environment. By connecting cellular behavior with treatment-associated changes, imaging supports development of therapies intended to improve tumor targeting and patient outcomes.