Mouse Kidney Isolation
Mouse Kidney Isolation - Related Videos
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
08:06
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
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Cited by 20 •
2011
In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.
06:56
Isolation of Double Negative αβ T Cells from the Kidney
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Cited by 26 •
2014
DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.
17:42
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
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Cited by 134 •
2011
Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes. Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.
08:30
Dissection and Culture of Mouse Embryonic Kidney
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Cited by 4 •
2017
This protocol describes a method for isolating and culturing metanephric rudiments from mouse embryos.
FAQs
The choice between removing one kidney or both affects how much tissue remains available for analysis and whether the study can examine a single organ or the complete renal pair. That decision should match the experimental goal, such as structural assessment, molecular profiling, cell dissociation, or organ culture, while preserving tissue needed for downstream work.
Preservation is not a generic final step: the medium should be compatible with the planned analysis. Tissue intended for histology or immunostaining requires preservation that supports structural and marker evaluation, whereas gene or protein analysis, cell dissociation, and organ culture may require different handling priorities. Matching preservation to the endpoint helps retain useful information.
Isolated kidneys provide more than tumor tissue alone because they retain the organ context in which disease-related changes occur. In cancer studies, investigators can examine renal tumors alongside tumor-associated changes and the surrounding kidney microenvironment. This supports analysis of how malignant cells relate to neighboring tissue and how treatment responses affect the organ.
An effective workflow proceeds in a defined sequence: humane euthanasia, entry through the abdominal cavity, careful separation of each kidney from surrounding tissues, and placement in an appropriate preservation medium. Careful dissection matters because the specimen must remain suitable for the selected structural, cellular, molecular, or functional analysis.
Planning begins with the intended readout. Histology and immunostaining emphasize preserved tissue structure and detectable markers; gene or protein analysis emphasizes molecular material; cell dissociation requires tissue that can be processed into cells; and organ culture requires material suitable for continued functional study. The isolation and preservation choices should therefore follow the endpoint.
These specimens can support studies of renal tumors, treatment responses, tumor-associated alterations, and interactions between malignant cells and the kidney microenvironment. Their value lies in connecting observations across levels: tissue structure, cellular composition, molecular signals, and function. Using the appropriate downstream assay allows investigators to relate a cancer-related change to its renal context.