Carbon 13
Carbon 13 - Related Videos
01:10
Carbon-13 (¹³C) NMR: Overview
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2024
Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
11:14
Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
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Cited by 6 •
2018
The fatty acid trophic marker approach, i.e., the assimilation of fatty acids as entire molecule and transfer into consumer tissue with no or minor modification, is hampered by knowledge gaps in fatty acid metabolism of small soil invertebrates. Isotopologue profiling is provided as a valuable tool to disentangle trophic interactions.
07:26
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Cited by 53 •
2012
13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.
![Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [<sup>13</sup>C]-octanoic Acid Breath Test](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F50301.jpg%3Fv%3D2026-08-29%252018%253A32%253A39&w=750&q=75)
12:24
Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
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Cited by 18 •
2013
Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.
13:59
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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Cited by 4 •
2013
Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.
FAQs
A nuclear spin of one-half allows Carbon-13 nuclei to be detected by nuclear magnetic resonance spectroscopy. Their signals reflect the chemical environments surrounding the carbon atoms, so the resulting spectrum provides structural information rather than merely indicating that carbon is present. This makes the isotope especially useful for examining the arrangement of atoms in carbon-containing compounds.
Carbon-13 has relatively low natural abundance, which contributes to comparatively simple NMR spectra. Fewer naturally occurring Carbon-13 nuclei produce less crowded spectral information, allowing chemists to distinguish signals associated with different chemical environments more readily. This simplicity supports structural interpretation when identifying carbon arrangements, functional groups, or reaction products.
Carbon-13 NMR can help identify the chemical environments of carbon atoms and use those differences to examine molecular structure. Chemists apply the technique to determine carbon connectivity, recognize functional groups, and assess whether a reaction produced the expected compound. The spectrum therefore connects observed signals with the structural features of the sample.
Carbon-13 labeling introduces a detectable carbon signature that can be followed as carbon-containing compounds change. In biochemistry, researchers use this approach to track the movement of carbon through metabolic pathways, linking an originating compound to later products or intermediates. The resulting information helps reveal how carbon is transformed within the pathway.
Chemists can use Carbon-13 labeling to follow carbon atoms during a chemical reaction, while Carbon-13 NMR can help examine the structures of resulting products. Together, these applications connect changes in carbon position or chemical environment with reaction outcomes. This evidence supports investigation of how carbon-containing compounds are transformed and which structural features appear afterward.
Carbon-13 methods support work across organic chemistry, biochemistry, and materials research. In organic chemistry, they help characterize compounds and products; in biochemistry, labeling follows metabolic carbon flow; and in materials research, labeled carbon can track material transformations. These uses extend the isotope from structural analysis to questions about chemical pathways and changing materials.