Induced Trophoblast Stem Cells
Induced Trophoblast Stem Cells - Related Videos
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Cited by 7 •
2010
In this video, we demonstrate the isolation of mouse blastocysts and the derivation of trophoblast stem cells from blastocysts. We also describe conditions for maintenance of the stem cell property as well as induction of differentiation in culture.
08:57
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Cited by 3 •
2016
Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.
08:21
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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Cited by 5 •
2017
Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
10:28
Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
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Cited by 44 •
2012
In this protocol, we describe the dissection of placentae from the mouse on pregnancy d10.5, followed by isolation of trophoblast cells using a Percoll gradient. We then demonstrate use of the isolated cells in a matrigel invasion assay.
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
FAQs
Defined transcriptional inputs help reset the identity of differentiated cells and activate trophoblast lineage programs. Culture conditions then support continued proliferation while preserving the cells’ capacity to produce specialized trophoblast subtypes. The outcome depends on maintaining both properties together: a self-renewing state for expansion and developmental potential for studying later stages of placental cell differentiation.
Self-renewal allows the population to proliferate so researchers can establish a controllable experimental system, whereas differentiation capacity preserves its developmental usefulness. If cells could expand but not produce specialized trophoblast subtypes, they would provide limited information about placental development. Maintaining both characteristics makes it possible to examine cell fate regulation across trophoblast lineage progression.
Reprogramming provides a way to examine how an established cellular identity can be reset toward a trophoblast lineage. This creates an experimental framework for investigating the transcriptional and environmental conditions associated with that transition. In developmental biology, the approach helps connect changes in cell identity with later effects on trophoblast development, implantation, and placental formation.
A typical workflow begins with differentiated cells, exposes them to defined transcriptional and culture conditions, and maintains the resulting population under conditions that support continued proliferation. Researchers can then assess whether the cells retain trophoblast stem cell properties and examine their ability to differentiate into specialized trophoblast subtypes. This sequence links induction, expansion, and developmental testing.
They provide a controllable cell-based system in which researchers can study trophoblast identity, self-renewal, and differentiation without relying only on a fixed developmental endpoint. Because trophoblasts contribute to fetal components of the placenta, the model can be used to investigate processes relevant to placental development and implantation, as well as the regulation of cell fate.
Induced trophoblast stem cells may help researchers examine how altered trophoblast development or cell fate regulation relates to pregnancy complications. Their expandable state can support controlled investigation of developmental mechanisms and comparison of differentiation outcomes. The same platform may also contribute to disease-mechanism studies, screening applications, or exploratory regenerative research, although these uses remain application areas rather than guaranteed outcomes.