Airway Epithelial Model
Airway Epithelial Model - Related Videos
09:26
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
0 Views •
Cited by 7 •
2015
Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.
11:21
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
0 Views •
Cited by 48 •
2010
This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.
12:31
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
0 Views •
Cited by 8 •
2016
This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.
10:18
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
0 Views •
Cited by 16 •
2019
We present a protocol to make dynamic measurements of the airway surface liquid pH under thin film conditions using a plate-reader.
11:26
Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
0 Views •
Cited by 17 •
2015
Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.
FAQs
At an air-liquid interface, airway cells can organize into basal, ciliated, and mucus-producing populations rather than remaining as an undifferentiated culture. This organization creates a tissue-like lining in which barrier behavior, mucus production, and ciliary clearance can be examined together. That added structure helps connect cellular responses to respiratory tract function.
The model links epithelial barrier activity with immune and host defense signaling. Investigators can examine how airway cells respond to inflammatory stimuli and how those responses relate to pathogen attachment or replication. This makes it possible to study epithelial contributions to infection without treating the respiratory lining as only a passive surface.
Mucus and ciliary clearance provide complementary defense processes that can be assessed alongside microbial interactions. A study can therefore ask not only whether a microbe attaches or replicates, but also how the organized epithelial surface responds. In infection research, this supports a more integrated analysis of early host defense and pathogen behavior.
Researchers typically culture airway cells at an air-liquid interface and allow them to differentiate into the principal epithelial populations. The resulting tissue-like system can then be exposed to microbes or inflammatory stimuli. Investigators examine pathogen attachment, replication, epithelial signaling, mucus and ciliary clearance, or interactions with immune cells, depending on the study question.
The system can provide evidence about several stages of host-pathogen interaction, including microbial attachment and replication, as well as epithelial signaling during challenge. It can also reveal changes relevant to mucus and ciliary clearance and show how epithelial cells interact with immune cells. These readouts connect local tissue behavior with broader infection mechanisms.
Researchers may choose an airway epithelial model when they need a controlled respiratory-tissue platform for studying disease mechanisms, antiviral responses, or potential therapeutics. Its value lies in combining tissue-like epithelial organization with defined exposure to microbes or inflammatory stimuli. In immunology and infection, that design helps separate epithelial effects from other contributors to disease.