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Immunology and Infection

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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Article DOI: 10.3791/52651 • 09:26 min • February 20th, 2015
February 20th, 2015 •
*1, *2, 2, 1, 1, 2, 1
1Departments of Pediatrics and Pharmacology, University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital, Third Military Medical University
* These authors contributed equally

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Summary
Automatic Translation

Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

Tags

Lymphocytic Microparticles Proapoptotic Effect Airway Epithelial Cells Cell Membrane-derived Vesicles MPs Plasma Membrane Shedding T Lymphocyte-derived Microparticles Actinomycin D Differential Centrifugation Flow Cytometry Cell Death Detection Bronchial Epithelial Cells Respiratory Bronchial Tissue Explants Disease Models Pharmacology Testing Toxicology Testing Airway Tract Tissue
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