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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
 

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Article doi: 10.3791/52651
February 20th, 2015 •
*1, *2, 2, 1, 1, 2, 1
1Departments of Pediatrics and Pharmacology, University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital, Third Military Medical University
* These authors contributed equally

Summary
Automatic Translation
February 20th, 2015

Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

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