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Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

8,658 Views

12:31 min

May 05, 2016

DOI:

12:31 min
May 05, 2016

8645 Views
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Summary

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This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.

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