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May 05, 2016
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This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.
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Cite this Article
Shojaie, S., Lee, J., Wang, J., Ackerley, C., Post, M. Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds. J. Vis. Exp. (111), e54019, doi:10.3791/54019 (2016).
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