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JoVE Journal
Bioengineering

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Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
 

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Article DOI: 10.3791/54019
May 5th, 2016

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Summary May 5th, 2016

This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.

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