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Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
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Bioengineering
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JoVE Journal Bioengineering
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

DOI:
10.3791/54019-v

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12:31 min

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May 05, 2016

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, , , ,
1Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning,Hospital for Sick Children

Chapters

  • 00:05Title
  • 00:47Scaffold Preparation
  • 03:57Thick Section Generation
  • 07:17Decontamination of Scaffold Sections and Recellularization Setup
  • 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
  • 11:52Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.

Tags

Keywords: Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution

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