Calcium-induced Signaling
Calcium-induced Signaling - Related Videos
02:11
Imaging Nicotine-Induced Calcium Signaling along Ventral Hippocampal Axons in Synaptic Co-Cultures
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2025
This video demonstrates how nicotine-mediated activation of nicotinic acetylcholine receptors in the mouse ventral hippocampus results in sustained calcium signaling along the axons.
12:19
Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
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Cited by 7 •
2015
We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.
08:14
Assessing T-cell Receptor-Induced Calcium Influx Using a Calcium Indicator Dye
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2025
This video analyzes T-cell receptor-induced calcium influx in T-cells. Anti-CD3 antibodies activate the T-cell signaling pathways, leading to an increase in intracellular calcium ions that bind with Indo-1 dye. In flow cytometry, a spectrum shift from the calcium-free state to the calcium-bound state correlates with T-cell receptor-induced calcium influx.
10:46
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Cited by 25 •
2013
Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.
08:21
Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
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Cited by 9 •
2017
This protocol outlines a simple method for analyzing calcium signals in plants generated by feeding hemipteran insects, such as aphids. Arabidopsis thaliana transformed with the GFP calcium biosensor GCaMP3 allow for the real-time in vivo imaging of calcium dynamics with a high temporal and spatial resolution.
FAQs
The initiating stimulus helps determine the route of calcium elevation. Receptor activation or membrane depolarization can open calcium channels, permitting Ca2+ to enter the cytoplasm. Signaling can also involve release from intracellular stores. Distinguishing these routes helps researchers relate the initiating cellular event to the subsequent immune or physiological response.
Calcium-binding proteins act as intracellular translators of altered Ca2+ concentrations. After binding calcium, they can influence enzyme activity, gene expression, secretion, or cytoskeletal behavior. This coupling explains how a change in calcium concentration becomes a functional response rather than remaining only an ion movement across a membrane or storage compartment.
Membrane events such as receptor activation or depolarization initiate calcium changes, while calcium-binding proteins connect those changes to downstream targets. The resulting effects can include altered gene expression and cytoskeletal behavior, linking signals at the cell surface with changes in cellular organization and longer-lasting functional responses.
Pathogens can alter host-cell calcium dynamics to influence cellular functions controlled by this pathway. Such manipulation may affect immune regulation or other host responses that depend on calcium-dependent changes in enzyme activity, gene expression, secretion, or cytoskeletal behavior. Studying these effects helps clarify how infection disrupts normal host-cell communication.
In immunology and infection, calcium signaling contributes to lymphocyte activation, phagocytosis, cytokine production, and antimicrobial responses. Examining these activities shows how intracellular calcium changes influence both immune-cell activation and the execution of defense functions. It also provides a framework for connecting disrupted calcium regulation with altered responses during infection.
The pathway is relevant because both immune cells and pathogens can influence calcium-dependent cellular functions. Research can therefore examine calcium signaling as a connection between host defense, immune regulation, and pathogen-driven interference. This perspective may help identify potential therapeutic targets aimed at modifying harmful signaling changes or supporting effective antimicrobial responses.