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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
JoVE Journal
Biology
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JoVE Journal Biology
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
DOI:

10:46 min

July 16, 2013

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Chapters

  • 00:05Title
  • 02:38Isolation of Corneal Endothelial Cells
  • 04:56Culture of Bovine Corneal Endothelial Cells
  • 05:56Mechanical Stimulation for Inducing Calcium Wave
  • 07:44Results: Calcium Wave Propagation in Bovine Corneal Endothelial Cells
  • 09:39Conclusion

Summary

Automatic Translation

Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.

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