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JoVE Journal
Biology

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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers
 

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Article DOI: 10.3791/50443 10:46 min
July 16th, 2013

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Summary July 16th, 2013

Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.

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