Intracranial Injection
Intracranial Injection - Related Videos
08:47
Intracranial Injection of Adeno-associated Viral Vectors
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Cited by 56 •
2010
Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
06:44
Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging
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Cited by 14 •
2020
Intracranial brain metastasis modeling is complicated by an inability to monitor tumor size and response to treatment with precise and timely methods. The presented methodology couples intracranial tumor injection with magnetic resonance imaging analysis, which when combined, cultivates precise and consistent injections, enhanced animal monitoring, and accurate tumor volume measurements.
02:25
Synergistic Effect of Intracranial and Intraperitoneal Delivery of Analgesic Drugs on Thermal Pain Response in a Rat
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2025
Source: Martinez, E., et al. Intracranial Pharmacotherapy and Pain Assays in Rodents. J. Vis. Exp. (2019).This video demonstrates the intracranial administration of an analgesic drug in an anesthetized rat with a pre-implanted guide cannula in the prefrontal cortex. An additional analgesic drug is co-administered intraperitoneally. Following recovery, the rat’s thermal pain response is assessed using Hargreave’s apparatus, where paw withdrawal latency is measured to evaluate the analgesic...
08:11
A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice
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Cited by 14 •
2011
In order to evaluate novel therapeutic paradigms for the treatment of glioma, physiological relevant models are essential. We utilize an implantable guide screw procedure for establishment of intracranial xenograft models that is more rapid and safer than stereotactic approaches.
FAQs
Direct CNS delivery helps investigators examine pathogen behavior after it reaches a neural site, rather than depending on the pathogen to cross biological barriers following peripheral exposure. This distinction supports focused analysis of neuroinvasion, tissue tropism, and infection-associated inflammation. It also makes local immune responses easier to characterize when those responses might be difficult to separate from effects occurring elsewhere in the body.
Stereotactic coordinates identify the intended anatomical site, while a fine needle directs the inoculum toward that location. Together, they improve control over where the substance is delivered and help limit injury to nearby tissue. Accurate placement is especially important when researchers want to relate pathogen distribution or local immune responses to a defined brain region or surrounding compartment.
A measured inoculum helps keep the administered challenge defined across experiments, making biological responses easier to interpret. Minimizing damage from needle placement is also essential because tissue injury could influence local inflammation independently of the infectious or experimental substance. Considering both factors helps researchers distinguish responses associated with the administered material from those associated with the procedure itself.
The target compartment changes the biological context in which the administered substance is encountered. Delivery into brain tissue can support analysis of responses within a selected neural site, whereas delivery into a surrounding compartment allows investigation of processes occurring adjacent to the brain. This distinction can help align the experimental model with questions about pathogen localization, tissue tropism, or local immunity.
The procedure generally includes anesthesia, stereotactic positioning, selection of anatomical coordinates, and advancement of a fine needle to the intended site. A measured inoculum is then administered, with care taken to limit damage to adjacent tissue. These steps create a controlled delivery process suitable for comparing CNS responses to defined substances or infectious challenges.
In this field, the technique can model pathogen entry into the CNS, investigate neuroinvasion and tissue tropism, and characterize immune responses at the site of infection. It also supports evaluation of antimicrobial interventions and examination of inflammation associated with infection. These applications connect the delivery site with outcomes that may be difficult to study after exposure outside the CNS.