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Intracranial Injection of Adeno-associated Viral Vectors

Article DOI: 10.3791/2140 • 08:47 min • November 17th, 2010
November 17th, 2010 •
1, 1
1Neurobiology and Anatomy, University of Rochester

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Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.

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Intracranial Injection Adeno-associated Viral Vectors Labeling Technique Visualization Specific Subsets Of Cells Brain Regions In Vivo Brain Sections Fluorescent Protein Viral Labeling Individual Cell Types Transgenic Mouse Lines AAV Vector Stereotaxic Coordinates Micropipette Automated Pump Precise Delivery Minimal Damage Injection Parameters Animal Age At Injection Injection Location Volume Of Injection Rate Of Injection AAV Serotype Promoter Driving Gene Expression Virally-induced Transgene Expression Visualization Of Cells And Processes Dendritic Spines
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